Given the importance of the complement anaphylatoxins in cellular recruitment during infection, the ability of secreted products from larval stages of Brugia malayi and Trichinella spiralis to influence C5a-mediated chemotaxis of human peripheral blood granulocytes in vitro was examined. Secreted products from B. malayi microfilariae almost completely abolished chemotaxis. This inhibition was b...

A comparative evaluation of four conservation chemicals (96% ethanol, 1% sodium benzoate, 10% formalin and glycerol), used to preserve Trichinella spiralis larvae's over 120 days, was performed. The results obtained after conservation with 96% ethanol, 1% sodium benzoate and glycerol showed no statistically significant differences in both the shape and the diameter in coiled Trichinella spirali...

Seaweeds are established sentinels for metal contamination and are utilised for biomonitoring. Metallothionein (MT) is a protein that is induced by metal exposure, and has been widely used as a biomarker for metal pollution. MT has not been reported in spiral wrack (Fucus spiralis), but has been identified in bladder wrack (Fucus vesiculosus), where it has been suggested to be a protective mech...

two isolates of trichinella were obtained from the jackal, canis aureus: one from the north caspian area, the other from the south-west khuzestan area of iran. the infectivity of the isolates was tested in laboratory animals, in a wild rodent (tatera indica) and in wild pigs. the isolate from khuzestan was of low infectivity to albino rats and it was provisionally identified as trichinella nels...

PREMISE OF THE STUDY Ficinia spiralis (Cyperaceae) is a declining sand-binding sedge of ecological and cultural importance. Microsatellite primers were developed in F. spiralis to investigate how population genetic structure is related to the pronounced morphological, physiological, and ecological variation observed in this species. METHODS AND RESULTS A 454 shotgun-sequencing approach was us...

Cell-mediated immunity during the muscular phase of Trichinella infection in humans was studied. Cell proliferation, the phenotypic changes in the T-cell population, and expression and production of cytokines were examined by using peripheral blood mononuclear cells (PBMC) collected at different times postinfection from 10 individuals who had acquired Trichinella spiralis and five individuals w...

Mast cells (MCs) deficient rats (Ws/Ws) were used to investigate the roles of MCs in visceral hyperalgesia. Ws/Ws and wild control (+/+) rats were exposed to T. spiralis or submitted to acute cold restraint stress (ACRS). Levels of proteinase-activated receptor 2 (PAR2) and nerve growth factor (NGF) were determined by immunoblots and RT-PCR analysis, and the putative signal pathways including p...