Background: Acanthamoeba is a free living ubiquitous protozoa, capable of causing Acanthamoeba keratitis (AK) of eyes and Acanthamoeba meningitis/ meningoencephalitis (AME) of central nervous system in human. Identification of amoeba is possible by a light microscope but, due to its poor resolution power, it is insufficient to distinguish the intimate sub cellular structures. As the resolution ...

Selenidium is a genus of gregarine parasites that infect the intestines of marine invertebrates and have morphological, ecological, and motility traits inferred to reflect the early evolutionary history of apicomplexans. Because the overall diversity and phylogenetic position(s) of these species remain poorly understood, we performed a species discovery survey of Selenidium from tube-forming po...

It is needless to describe the chemical nature of the drug here and the literature on the subject of the treatment of malaria by the use of plasmochin is now too extensive to refer to in detail. The observations made by others may be summarised as follows :? (1) It has a destructive action on the trophozoites of P. vivax and P. malarice. (2) It has no such action on the trophozoites of P. falci...

Based on a serum-free and CO,-independent culture of Toxoplasma go&ii in vitro, a simple single arrangement mass production of trophozoites of the highly virulent BK strain of this parasite was developed. Starting simultaneously, lo7 host cells (HEp-2) and 10’ Toxoplasma trophozoites were cultured in one cell-factory@ for 5 days, removing unattached cells, cell remnants and extracellular parasi...

A total of 618 sera from inhabitants living in various endemic areas in Indonesia were examined for IgG against Plasmodium falciparum utilizing young trophozoites and mature schizonts as antigens by the method of ELISA and IFAT. In general, antibodies against trophozites (RESA) based on ELISA and antibodies against schizonts based on IFAT showed a correlation of malarial antibodies with the lev...

Hemoglobinopathies have a protective role in malaria that appears to be related to alterations in red blood cell (RBC) properties. Thalassemic RBCs infected with Plasmodium falciparum showed greatly reduced cytoadherence and rosetting properties as well as impaired growth and multiplication. A significant decrease in the levels of falciparum antigens associated with the membrane of infected 8-t...

Materials and methods: In this study a total of 17 trophozoite samples, obtained either directly from stool samples or after excystation, or by duodenal aspiration, were used. A set of primers was chosen to amplify the different regions of triose phosphate isomerase (tpi) and a segment of the glutamate dehydrogenase (gdh) genes. A single-stranded conformational polymorphism technique was also u...

A case of Pneumocystis carinii pneumonia developing a patient with treated diffuse lymphocytic lymphoma is described. The electron microscopic features and life cycle of the organism are illustrated. The patient died twenty-four hours after the biopsy had been taken. Septrin appeared to have affected the trophozoite stage. Pneumocystis carinii appears to induce interstitial pulmonary oedema and...