نتایج جستجو برای: trizol volume
تعداد نتایج: 315283 فیلتر نتایج به سال:
The reliability of gene expression profiling, based technologies and methods to find transcriptional differences representative of the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...
Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell lysis are fundamental steps and of great...
Isolating RNA from insects is becoming increasingly important in molecular entomology. Four methods including three commercial kits RNeasy Mini Kit (Qiagen), SV Total RNA isolation system (Promega), TRIzol reagent (Invitrogen), and a cetyl trimethylammonium bromide (CTAB)-based method were compared regarding their ability to isolate RNA from whole-body larvae of Thaumatotibia leucotreta (Meyric...
Epidemiological and histopathological studies have indicated that proliferative inflammatory atrophy (PIA) of the prostate is closely associated with the onset and development of prostate cancer (PCa). However, accurate isolation of PIA still remains a difficult matter, as well as high-quality RNA extraction from isolated PIA. These issues generated a lack of molecular evidence to support the m...
RNA-based microbiological analyses, e.g., transcriptome and reverse transcription-quantitative PCR, require a relatively large amount of high quality RNA. RNA-based analyses on microbial communities in deep-sea hydrothermal environments often encounter methodological difficulties with RNA extraction due to the presence of unique minerals in and the low biomass of samples. In the present study, ...
This report describes a newly developed method using Trizol LS reagent that can reliably extract high quality total RNA from frozen human leukaemic bone marrow samples. Extraction of total RNA from 71 frozen leukaemic bone marrow samples obtained at the time of diagnosis produced a median yield of 145 micro g/ml leukaemic bone marrow. Total RNA samples could be reverse transcribed into cDNA and...
Analysis of Env RNA expression in lung and airways Total RNA was isolated from lung, from trachea and from epithelial tissue scraped from the inside of the nose, by using a Polytron tissue homogenizer (Brinkmann) and Trizol RNA isolation reagent (Invitrogen). Samples were treated with DNase and with reverse transcriptase in the presence of a 3 0 Env primer; they were then subjected to 30 cycles...
ARTICLE INFO ABSTRACT Received: Accepted: Online: Jan 18, 2016 Apr 10, 2016 Apr 20, 2016 A high quality RNA (407-420μg/mL) was extracted from blood of sheep infected with Trichostrongylus spp., by using RBC’s lysis buffer followed by TRIzol reagent. Agarose gel electrophoresis showed clearly, discrete ribosomal RNA with no apparent degradation. The purified RNA samples were used successfully in...
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