Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells. The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 microg [dry weight] ml of extended boar semen(-1)). The same exposure concentration d...

Exposing a wild-type culture of Trichoderma harzianum to gamma irradiation induced two stable salt-tolerant mutants (Th50M6 and Th50M11). Under saline conditions, both mutants greatly surpassed their wild type strain in growth rate, sporulation and biological proficiency against Fusarium oxysporum, the causal agent of tomato wilt disease. Tolerant T. harzianum mutants detained a capability to g...

An isolate of Trichoderma harzianum Rifai from an infected cacao pod produces and secretes nonanoic (pelargonic) acid into a liquid culture medium. Nonanoic acid (NA) was very inhibitory to spore germination and mycelial growth of two cacao pathogens, Crinipellis perniciosa Stahel and Moniliophthora roreri Cif. H.C. Evans. It was highly active causing 75% inhibition of spore germination in an i...

The objectives of this study were to investigate whether seed biopriming through drought tolerant isolates of Trichoderma harzianum induces drought tolerance in wheat. Physiological and biochemical parameters were also monitored under greenhouse conditions to explore the mechanism underlying plant water stress resilience in response to Trichoderma inoculation.The impact of bioprospecting Tricho...

Impacts of metals to the growth and enzyme production of fungi were tested with Cd(0-10 mg kg–1), Co(0-100 mg kg–1), Cr(0-100 mg kg–1), Li(0-100 mg kg–1) or Mn(0-400 mg kg–1) containing media with ABTS [2,2’-azino-bis (3-ethylbentzthiazoline6-sulfonicacid)] as indicator of oxidative enzymes, laccase and/ or peroxidases. All ascomycetes Alternaria sp., Chaetomium sp., Epicoccum sp., Fusarium sp....

Two isolates of Trichoderma, which reduce the incidence of witches’broom disease caused in cocoa by Crinipellis perniciosa, were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, β-1,3-glucanase, total cellulase, endoglucanase, arylβ-glucosid...

The biological effectiveness of twenty-eight native isolates Trichoderma along with two known species (Trichoderma viride and harzianum) were tested against Rhizoctonia solani using dual culture technique. Of them, seven the in pot under net-house conditions wirestem disease cauliflower caused by R. solani. Inhibitory effects on statistically very significant for all Trichoderma. Thirteen exhib...

Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28 degree C in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylo...

Nine fungicides namely: carbendazim, hexaconazole, thiophonate methyl, triadimefan, metalaxyl, mancozeb, captan, copper oxychloride and chlorothalonil and seven bioagents namely: Trichoderma harzianum, Trichoderma viride, Gliocladium virens, Lacaria laccata, Boletus edulis, Suillus placids and Russula lutea were evaluated in vitro by using dual culture and cultural filtrate techniques for their...

We describe the second known case of human infection by Trichoderma harzianum. A disseminated fungal infection was detected in the postmortem examination of a renal transplant recipient and confirmed in culture. The only other reported infection by this fungus caused peritonitis in a diabetic patient. The in vitro antifungal susceptibilities of the clinical strain and three other strains of Tri...