Translocation of the mRNA-tRNA complex in the ribosome, which is catalyzed by elongation factor EF-G, is one of critical steps in the elongation cycle of protein synthesis. Besides this conventional forward translocation, the backward translocation can also occur, which can be catalyzed by elongation factor LepA. However, the molecular mechanism of the translocation remains elusive. To understa...

Our previous study showed differential subcellular localization of protein kinase C (PKC) delta by phorbol esters and related ligands, using a green fluorescent protein-tagged construct in living cells. Here we compared the abilities of a series of symmetrically substituted phorbol 12,13-diesters to translocate PKC delta. In vitro, the derivatives bound to PKC with similar potencies but differe...

The entry of exogenous fibroblast growth factor 2 (FGF-2) to the cytosolic/nuclear compartment was studied and compared with the translocation mechanism used by FGF-1. To differentiate between external and endogenous growth factor, we used FGF-2 modified to contain a farnesylation signal, a CaaX-box. Because farnesylation occurs only in the cytosol and nucleoplasm, farnesylation of exogenous FG...

Similarly to many protein toxins, the growth factors fibroblast growth factor 1 (FGF-1) and FGF-2 translocate from endosomes into the cytosol. It was recently found that certain toxins are dependent on cytosolic Hsp90 for efficient translocation across the endosomal membrane. We therefore investigated the requirement for Hsp90 in FGF translocation. We found that low concentrations of the specif...

introduction the lead is considered as one of the most toxic heavy metals in environment organizations of industrial and developing countries. therefore, refining of soil and water contaminated to this element is from most important environmental policies of this countries. lead metal is unnecessary for the body, so that trace amount in the body, indicating contamination with this element. lead...

in order to investigate the effect of cd and zn on uptake, concentration and the translocation factor of the cd and zn in the rice plant, a factorial experiment was conducted with four factors including two rice cultivars of vandana and hashemi, two waterlogged and non-waterlogged conditions and three levels of zn and cd (0, 5 and 10 mg kg-1 soil). the experiment was carried out in a randomized...

abstract the use of plants for remediation of heavy metals contaminated soils is an economical, cheap and effective strategy. this research was conducted in order to study the effect of potassium chloride on cadmium uptake and enhancement of it's phytoremediation by canola (brassica napus l.) and sunflower (helianthus annuus l.) in a cadmium polluted soil from angoran in zanjan province. in a r...

The adhesion and aggregation of platelets at sites of vascular injury is dependent on the initial binding of the GP Ib/V/IX receptor complex to immobilized von Willebrand factor (VWF). Under flow conditions, this interaction supports platelet translocation that is characteristically stop-start in nature. High resolution imaging of platelets during surface translocation on immobilized VWF reveal...

The adhesion and aggregation of platelets at sites of vascular injury is dependent on the initial binding of the GP Ib/V/IX receptor complex to immobilized von Willebrand factor (VWF). Under flow conditions, this interaction supports platelet translocation that is characteristically stop-start in nature. High resolution imaging of platelets during surface translocation on immobilized VWF reveal...

down’s syndrome, or 21 trisomy, is the most common autosomal abnormality, with incidence of 1 per 815 live births in iran. worldwide reports indicate that about 95% are regular trisomy, or nondisjunction, 1% are mosaic and 4% due to translocation. however, these values show a geographical variation. a cytogenetic study of down's syndrome, or trismoy 21, was carried out on 366 cases (202 males a...