background: bladder cancer (bc) is the most commonly diagnosed genitourinary cancer in iran, presented in both men and women. bc is a multifactorial trait resulting from the complex interaction between several genes and environmental factors. long stress-induced non-coding transcript 5 (lsinct5), a member of the long non-coding rnas, is abundantly expressed in high proliferative cells, as well ...

The recently published recommendations for the harmonization of methodology for BCR-ABL fusion transcript monitoring in chronic myeloid leukemia (CML) patients responding to treatment with tyrosine kinase inhibitors are timely and pertinent for all laboratories undertaking such testing.1 The authors provide practical guidelines for laboratories to help optimize their testing and reporting proce...

Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors of transcription factors composed of three family members: PPARα, PPARβ/δ and PPARγ. This study was aimed to evaluate the role of PPARs in the estradiol production via follicle stimulating hormone (FSH) in the ovine Sertoli cells. At the first step, transcripts of PPARα, PPARβ /δ and PPARγ were evaluated by quantitative r...

The level of transcripts encoding the skeletal muscle acetylcholine receptor (AChR) was determined during embryonic development in Xenopus laevis. cDNAs encoding the alpha, gamma, and delta subunits of the Xenopus AChR were isolated from Xenopus embryo cDNA libraries using Torpedo AChR cDNAs as probes. The Xenopus AChR cDNAs have greater than 60% amino acid sequence homology to their Torpedo ho...

The bioavailability of methylmercury (MeHg) in the brains of  orange spotted groupers, captured from four creeks of Mahshahr embayment was measured.  Then the effects of this pollutant on the regulation of gene expression, acetylcholinesterase transcript levels was chosen in order to monitor the amounts of methylmercury concentrations in the creeks, and the fluctuations of mRNA expressions in t...

RNA-binding proteins (RBPs) are pivotal in orchestrating several steps in the metabolism of RNA in eukaryotes thereby controlling an extensive network of RBP-RNA interactions. Here, we employed CLIP (cross-linking immunoprecipitation)-seq datasets for 60 human RBPs and RIP-ChIP (RNP immunoprecipitation-microarray) data for 69 yeast RBPs to construct a network of genome-wide RBP- target RNA inte...

Alterations in the pattern of epidermal differentiation and proliferation occur during mouse skin carcinogenesis. We have used cDNA clones corresponding to the major keratin subunits synthesized in differentiating epidermal cells (Mr 67,000 and 59,000) and in proliferating epidermal cells (Mr 60,000, 55,000, and 50,000) to study changes in keratin gene transcript levels in mouse epidermis expos...

Phytoene synthase (Psy) and Phytoene desaturase (Pds) are the first two regulatory enzymes in the carotenoids biosynthetic pathway. The genes Psy and Pds are under transcriptional control in many photosynthetic organisms. In the present study, using quantitative real time- PCR (qRT-PCR), the effects of uncoated and gum-Arabic coated PbS nanoparticles (GA-coated PbS NPs) and light intensity on t...

Identification of prognostic factors and risk-based post-remission therapy was proposed to improve the outcomes of acute myeloid leukemia (AML) and a mutation of FLT3 has been reported to be a risk factor, especially for pediatric patients. Recently, FLT3 expression level was implicated to have prognostic significance in adults, but little is known for childhood AML. To define the prognostic si...