نتایج جستجو برای: total cdna

تعداد نتایج: 837865  

Journal: :BioTechniques 2003
Tomoko Hirozane-Kishikawa Toshiyuki Shiraki Kazunori Waki Mari Nakamura Takahiro Arakawa Jun Kawai Michela Fagiolini Takao K Hensch Yoshihide Hayashizaki Piero Carninci

The normalization and subtraction of highly expressed cDNAs from relatively large tissues before cloning dramatically enhanced the gene discovery by sequencing for the mouse full-length cDNA encyclopedia, but these methods have not been suitable for limited RNA materials. To normalize and subtract full-length cDNA libraries derived from limited quantities of total RNA, here we report a method t...

Journal: :Acta veterinaria Scandinavica 2001
J Yao J L Burton P Saama S Sipkovsky P M Coussens

Recent developments in expressed sequence tag (EST) and cDNA microarray technology have had a dramatic impact on the ability of scientists to study the responses of thousands of genes to external stimuli, such as infection, nutrient flux, and stress. To date however, these studies have largely been limited to human and rodent systems. Despite the tremendous potential benefit of EST and cDNA mic...

Journal: :Nucleic acids research 1998
T Trenkle J Welsh B Jung F Mathieu-Daude M McClelland

A method is presented in which the reduced complexity and non-stoichiometric amplification intrinsic to RNA arbitrarily primed PCR fingerprinting (RAP-PCR) is used to advantage to generate probes for differential screening of cDNA arrays. RAP-PCR fingerprints were converted to probes for human cDNA clones arrayed as Escherichia coli colonies on nylon membranes. Each array contained 18 432 cDNA ...

Journal: :The Journal of biological chemistry 1979
G A Evans M G Rosenfeld

DNA complementary to prolactin-specific sequences was prepared using a method which did not require purification of the prolactin mRNA. cDNA was synthesized using total cytoplasmic mRNA from a prolactinproducing rat pituitary cell line (GH,) as a template for avian myeloblastosis virus reverse transcriptase. A cDNA species 840 nucleotides in length was purified by electrophoresis on formamide p...

Journal: :Nucleic acids research 1990
C Enders A Schlerf O Mell L I Grossman B Kadenbach

Mammalian cytochrome c oxidase (COX) is composed of three catalytic, on mitochondrial DNA encoded subunits, and ten nuclear encoded subunits, which have been ascribed a regulatory function (1). At least three nuclear encoded subunits occur in tissue-specific isoforms (Via, Vila and VIE). The liverand hearttype cDNA has been described for rat subunit Via (2) and bovine subunit Vm (3). The liver-...

Journal: :Nucleic acids research 2004
Pavel A Zhulidov Ekaterina A Bogdanova Alex S Shcheglov Laura L Vagner George L Khaspekov Valery B Kozhemyako Mikhail V Matz Ella Meleshkevitch Leonid L Moroz Sergey A Lukyanov Dmitry A Shagin

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation-reassociation of cDNA, degradation of the double-stranded (ds) fraction formed by abundant transcripts and PCR amplification of the equalized single-stranded (ss)...

Journal: :The Biochemical journal 1996
S Modaressi B Christ J Bratke S Zahn T Heise K Jungermann

In human liver, phosphoenolpyruvate carboxykinase (PCK; EC 4.1.1.32) is about equally distributed between cytosol and mitochondria in contrast with rat liver in which it is essentially a cytosolic enzyme. Recently, the isolation of the gene and cDNA of the human cytosolic enzyme has been reported [Ting, Burgess, Chamberlian, Keith, Falls and Meisler (1993) Genomics 16, 698-706; Stoffel, Xiang, ...

Journal: :Soil Biology & Biochemistry 2021

Both the frequency and intensity of drought events are expected to increase, with unresolved alterations peatland methane cycling involved microbial communities. While existing studies have assessed effects via experimental approaches under controlled conditions, our knowledge, no examined in-situ natural in restored temperate fens. In this study, we used quantitative polymerase chain reaction ...

Journal: :Indian journal of experimental biology 2008
Rekha Kansal Kalika Kuhar Isha Verma Ram Niwas Gupta Vijay Kumar Gupta Kirpa Ram Koundal

It has been difficult to extract a good quality total RNA from the plant parts (such as seeds) which contain high levels of phenolic compounds, carbohydrates and other compounds that bind and/or co-precipitate with RNA. A simple, rapid and efficient method for isolating total RNA from polyphenols and polysaccharide rich plant tissues has been developed. Seeds of leguminosae family were chosen f...

2012
John A Porco

Diversity-oriented synthesis (DOS) is an important field involving the synthesis of libraries of diverse small molecules for applications including biological screening. The last decade has witnessed major progress in the field, with continued emphasis on structural and functional diversity for DOS library construction. A number of recent reviews have updated the community on this topic, includ...

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