A model for the maturation of tomato spotted wilt virus (TSWV) particles is proposed, mainly based on results with a protoplast infection system, in which the chronology of different maturation events could be determined. By using specific monoclonal and polyclonal antisera in immunofluorescence and electron microscopy, the site of TSWV particle morphogenesis was determined to be the Golgi syst...

Tomato yellow leaf curl virus (TYLCV) and tomato spotted wilt virus (TSWV) are common diseases of tomato in Florida. TYLCV occurs in central and southern Florida whereas TSWV occurs in northern Florida and throughout all states of the southeastern USA. TYLCVresistant cultivars and advanced breeder’s varieties are available for commercial use, but vegetable producers don’t often use them. A vari...

BACKGROUND Tomato spotted wilt virus (TSWV) has a very wide host range, and is transmitted in a persistent manner by several species of thrips. These characteristics make this virus difficult to control. We show here that the over-expression of the mitochondrial alternative oxidase (AOX) in tomato and petunia is related to TSWV resistance. RESULTS The open reading frame and full-length sequen...

Complementary DNA to the genomic RNA of tomato spotted wilt virus (TSWV) was synthesized and cloned in either pUC19 or lambda gt10. Restriction endonuclease maps were constructed from cDNA clones specific for the S and the M RNA segments, extending 3.0 and 5.4 kbp respectively. The nucleotide sequences of the 3' and 5' termini of both RNA molecules were determined. The S and M RNAs contain inve...

Current GM crops are thus far the almost exclusive domain of herbicide and insect resistance traits. The Bt toxins used for insect control have a narrow specificity against lepidopteran and coleopteran pests only. Yet, aphids and thrips are highly important pests worldwide, causing severe direct losses and transmitting devastating viruses such as Tomato Spotted Wilt Virus (TSWV). So far, few us...

BACKGROUND Spotted wilt, caused by tomato spotted wilt virus (TSWV), has been one of major diseases in cultivated peanut grown in the southeastern United States (US) since 1990. Previously a major quantitative trait locus (QTL) controlling spotted wilt disease resistance was mapped to an interval of 2.55 cM genetic distance corresponding to a physical distance of 14.4 Mb on chromosome A01 of pe...

A severe disease of tomato was observed in 2010 in a greenhouse in the province of Lecce (Apulia, southern Italy). Plants showed interveinal yellowing and thickening of mature leaves and a bushy appearance of the new growth. Pale-yellow spots, which became sunken and necrotic, were scattered on the fruit surface. Leaf symptoms were reminiscent of those induced by Tomato chlorosis virus (ToCV) o...