نتایج جستجو برای: tof ms

تعداد نتایج: 132215  

Journal: :Frontiers in microbiology 2016
Kaveh Emami Andrew Nelson Ethan Hack Jinwei Zhang David H. Green Gary S. Caldwell Ehsan Mesbahi

The genus Pseudoalteromonas constitutes an ecologically significant group of marine Gammaproteobacteria with potential biotechnological value as producers of bioactive compounds and of enzymes. Understanding their roles in the environment and bioprospecting for novel products depend on efficient ways of identifying environmental isolates. Matrix Assisted Laser Desorption/Ionization-Time of Flig...

2012
Nicole Strepparava Helmut Segner Orlando Petrini

Flavobacteriosis, in Switzerland caused mainly by Flavobacterium psychrophilum (Flavobaceriaceae), has become an important problem for the Swiss fish farming industry. While records of skin and gill infections have been described since a long time, systemic infections, known as the so-called rainbow trout fry syndrome (RTFS), have been reported for the first time not until 1992. Since then, occ...

2006
T. Dickel M. Petrick W. R. Plaß

A multiple-reflection time-of-flight mass spectrometer (MR-TOF-MS) has been developed, in which isobaric ions can be separated and measured with a cycle time of about 1 ms. It is based on a principle proposed by Wollnik et al. [1] and on an ion-optical reflector design developed by Verentchikov et al. [2]. The layout of the MR-TOF-MS is shown schematically in Fig. 1. In its present configuratio...

2017
Atang Bulane Anwar Hoosen

Background Rapid and accurate identification of pathogens is of utmost importance for management of patients. Current identification relies on conventional phenotypic methods which are time consuming. Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) is based on proteomic profiling and allows for rapid identification of pathogens. Objective We compare...

2017
Sirama Niare Lionel Almeras Fatalmoudou Tandina Amina Yssouf Affane Bacar Ali Toilibou Ogobara Doumbo Didier Raoult Philippe Parola

BACKGROUND Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens a...

Journal: :Journal of chromatography. A 2005
Xueguo Chen Liang Kong Xingye Su Chensong Pan Mingliang Ye Hanfa Zou

An approach for the separation and identification of components in a traditional Chinese medicine Psoralea corylifolia was developed. Ion-exchange chromatography (IEC) was applied for the fractionation of P. corylifolia extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. Each of the enriched fractions was then further separated on an ODS column with d...

2017
Jing Xu Bin Xu Chuanhao Tang Xiaoyan Li Haifeng Qin Weixia Wang Hong Wang Zhongyuan Wang Liangliang Li Zhihua Li Hongjun Gao Kun He Xiaoqing Liu

Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to ...

2010
Clifton K. Fagerquist Omar Sultan

A method has been developed to identify the α-subunit of Shiga toxin 2 (α-Stx2) from Escherichia coli O157:H7 using matrix-assisted laser desorption/ionization time-of-flight-time-of-flight tandem mass spectrometry (MALDI-TOF-TOF-MS/MS) and top-down proteomics using web-based software developed in-house. Expression of Stx2 was induced by culturing E. coli O157:H7 on solid agar supplemented with...

Journal: :Journal of clinical microbiology 2013
Jonathan H K Chen Pak-Leung Ho Grace S W Kwan Kevin K K She Gilman K H Siu Vincent C C Cheng Kwok-Yung Yuen Wing-Cheong Yam

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of bacteria and fungi was recently introduced in microbiology laboratories. This technology could greatly improve the clinical management of patients and guidance for chemotherapy. In this study, we used a commercial MALDI Sepsityper extraction method to evaluate the performance of...

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