Background: Ananas comosus, commonly known as pineapple, is a fruit that is endorsed by the Ministry of Agriculture to be economically important to the island. Tissue culture maybe used as an additional method of propagation to supplement traditional methods to increase productivity. This study was therefore designed to assess the effectiveness of utilising tissue culture techniques to produce ...

NOT many years ago tissue culture techniques were practised in only a small number of highly specialized laboratories. Today the position has changed completely, and there is hardly a field in biological and medical researchwhere these techniques are not finding some application. The supply of suitable textbooks has not managed to keep abreast of this rapid expansion and Dr. Paul's "Cell and Ti...

There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D Tissue (RAFT™) culture system in terms of viability and hormone production. H...

due to the number of new substances coming into use every year and the increasing amounts of chemicals, which are introduced into the environment, there is a high demand for a rapid, reliable and cost-effective method for detection of developmental toxicity. to meet this challenge various in vitro techniques have been established additional to in vivo animal testing. this review introduces the ...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

SUMMARY Virus-like particles were recently described in an ependymoblastoma of CS7BL/J mice. For characterization of these particles, the tumor was explanted into cell culture. This study reports the growth of these cells in culture and the persistence of virus-like particles in the cells for up to 5 months in culture. The particles correspond to intracyto plasmic A particles and extracellular ...

Splenic lymphocytes were collected from BCG-vaccinated ddY mice and cultured with or without the antigen. Thus obtained supernatants were tested by tissue culture techniques. Normal mouse peritoneal macrophages were infected with M. lepraemurium in vitro and the effects of MycolF (mycobacterial growth inhibiting factor) in these supernatants on the growth of the bacilli were examined. The multi...

Animal and two-dimensional cell culture models have had a profound impact on not only lung research but also medical research at large, despite inherent flaws and differences when compared with in vivo and clinical observations. Three-dimensional (3D) tissue models are a natural progression and extension of existing techniques that seek to plug the gaps and mitigate the drawbacks of two-dimensi...

AIMS To improve detection of neuroblastoma metastases in the bone marrow: morphological evaluation of bone marrow is a routine and important component of the clinical staging of neuroblastoma and it depends on the successful identification of tumour cells which may only be present at extremely low levels. METHODS Bone marrow mononuclear cells from patients with neuroblastoma were incubated in...