Vanadium is a hard, silver-grey transition metal found in at least 60 minerals and fossil fuel deposits. Its oxide other vanadium salts are toxic to humans, but the effects depend on form, dose, exposure duration, route of intoxication. used by some life forms as an active center enzymes, such bromoperoxidase ocean algae nitrogenases bacteria. The structure biochemistry vanadate resemble those ...

Diabetic wound, also known as diabetic foot ulcer (DFU), is a form of chronic lesion that disrupts the patient's lifestyle and, in severe cases, may be fatal. Recent endeavors utilizing enzymes, particularly nanozymes conjunction with other materials, have revealed potential to treat wounds. Herein, rational structural design enzyme‐based biomaterials summarized after presenting types and proce...

Neuroblastoma cells can acquire resistance mechanisms that make them invulnerable to chemotherapeutic agents. The use of nanoparticles as drug carriers provides the possibility deliver several drugs simultaneously specific tumoral cell populations, improving their therapeutic outcome. Herein, development a multifunctional nanoplatform based on assembly protocells (PC) and polymeric nanocapsules...

Gamma irradiation is used on Penicillium cyclopium in order to obtain mutant cells of high L-asparaginase productivity. Using gamma irradiation dose of 4 KGy, P. cyclopium cells yielded L-asparaginase with extracellular enzyme activity of 210.8 ± 3 U/ml, and specific activity of 752.5 ± 1.5 U/mg protein, which are 1.75 and 1.53 times, respectively, the activity of the wild strain. The enzyme wa...

Lysosomal storage diseases (LSDs) are a group of inherent diseases characterized by massive accumulation of undigested compounds in lysosomes, which is caused by genetic defects resulting in the deficiency of a lysosomal hydrolase. Currently, enzyme replacement therapy has been successfully used for treatment of 7 LSDs with 10 approved therapeutic enzymes whereas new approaches such as pharmaco...

The intracellular delivery of enzymes is an essential methodology to extend their therapeutic application. Herein, we have developed dissociable supermolecule-enzyme polyelectrolyte complexes based on reduction-cleavable cationic polyrotaxanes (PRXs) for the reactivation of delivered enzymes. These PRXs are characterized by their supramolecular frameworks of a polymeric chain threading into cyc...

hiv-1 integrase (in) enzyme, one of the three main enzymes of hiv-1, catalyzed the insertion of the viral dna into the genome of host cells. because of the lack of its homologue in human cells and its essential role in hiv-1 replication, in inhibition represents an attractive therapeutic target for hiv-1 treatment. since identification of in as a promising therapeutic target, a major progress h...

Antimicrobial resistance (AMR) occurs when microorganisms fail to respond the therapeutic onslaught of antibiotics. Extended-spectrum beta-lactamase (ESBL) and AmpC enzymes are important AMR mechanisms that erode efficacy Here, we report on detection susceptibility ESBL- AmpC-producing bacteria from livestock poultry environments. Bacteriological molecular biology tools were used for isolation ...