Microbial release of greenhouse gases from thawing permafrost is a global concern. Seventy-six metagenomes were generated from low-soil-organic-carbon mineral cryosols from Axel Heiberg Island, Nunavut, Canada, during a controlled thawing experiment. Permafrost thawing resulted in an increase in anaerobic fermenters and sulfate-reducing bacteria but not methanogens.

This study aimed to investigate the effect of storage at ?3 °C on myofibrillar protein in fast or slow frozen pork. Five pork loins 48 h post-mortem were subjected either (cold metal plate/?80 °C) freezing (still air/?20 followed by for 0, 1, 3, and 7 days before thawing. Freezing rate significantly influenced proteins within 3 °C, evidenced higher thaw loss, surface hydrophobicity reduced wate...

In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots: non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and vi...

The resistance of concrete structures to the impact cyclic freezing and thawing is one key long-term characteristics, which further determines operation its service life. To date, alternating freeze-thawing cycles under various operating conditions has been little studied related several manufacturing processes: simple vibrated, variotropic centrifuged, improved vibrocentrifuged. purpose this s...

We studied the effect of freezing and thawing of serum on the determination of lipoprotein(a) [Lp(a)] with a commercial enzyme-linked immunosorbent assay (ELISA) and an immunoturbidimetric assay (ITA). Portions of sera from 11 apparently healthy persons and pooled sera, from an additional 10 subjects were frozen at either -20 or -70 degrees C and thawed at room temperature. Cycles of freezing a...

A total of 30 ejaculates from three Hampshire crossbred boars were used to study the effect of freezing rates and thawing methods on quality of frozen boar semen. Semen was frozen using three freezing rates-30 0 c/min, 50 0 c/min and 70 0 c/min in a programmable biological semen freezer. The semen characteristics viz., sperm motility, live sperm, HOST reacted spermatozoa and intact acrosome wer...

Some biosamples obtained from biobanks may go through thawing before processing. We aim to evaluate the effects of thawing at room temperature for different time periods on gene expression analysis. A time course study with four time points was conducted to investigate the expression profiling on 10 thawed normal mice renal tissue samples through Affymetrix GeneChip mouse gene 2.0 st array. Mic...

BACKGROUND Transfusion of plasma is a basic treatment for complex coagulopathies as well as in major blood loss. Early transfusion of plasma after trauma with major hemorrhage has been recommended by retrospective studies. However, the use of plasma is often hampered by the need to maintain a cold chain and the time needed for thawing fresh-frozen plasma (FFP). With freeze-dried (lyophilized) p...

Retail stores often offer discounts on purchases of large amounts of meat. Some of the meat is prepared the same day, and some is stored for later use. This study evaluates the effect of retail packaging, freezing and thawing on the quality of beef patties. Loosely ground beef was retail-packed in: (1) highly permeable wrap, and (2) high oxygen MAP. The meat was subsequently refrigerated at 4°C...