AIM To assess the effect of the growth promoter avilamycin on emergence and persistence of resistance in enteric bacteria in the pig. METHODS AND RESULTS Pigs (treated with avilamycin for 3 months and controls) were challenged with multi-resistant Salmonella Typhimurium DT104 and faecal counts were performed for enterococci, Escherichia coli, S. Typhimurium and Campylobacter (before, during a...

The emergence of multidrug resistance has become an environmental safety issue worldwide concern. Numerous studies have confirmed that heavy metals can promote the spread bacterial resistance. However, in addition to co-resistance plasmids, role and mechanism at sublethal levels (< minimum inhibitory concentration [MIC]) on antibiotic remain poorly defined. Here we investigated alteration pheno...

The aim of this study was to investigate the pathogenic potential and antibiotic resistance 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) drinking water 28) sold in city Maputo, Mozambique. were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs performed targeting five virulence genes (stx, lt, st, astA, eae) three groups a...

INTRODUCTION This study aimed to determine the drug susceptibility patterns and genetic elements related to drug resistance in isolates of Salmonella enterica serovar Typhi (S. Typhi) from the Faisalabad region of Pakistan. METHODOLOGY The drug resistance status of 80 isolates were evaluated by determining antimicrobial susceptibility, MICs, drug resistance genes involved, and the presence of...

The aims of the current study were to detect the virulence factors and antibiotic resistance of Shiga toxin-producing E. coli, in animal milk and dairy products in Iran. After E. coli dentification with culture method, PCR assay were developed for detection of pathogenic genes, serotypes and antibiotic resistance genes of E. coli. Results showed that out of 719 samples, 102 (14.18%) were confir...

The occurrence of antibiotics and antibiotic resistance genes (ARGs) was quantified in water and sediment samples collected from a 72 km stretch of the Haihe River, China. Tetracycline resistance genes (tetW, tetQ, tetO, tetT, tetM, tetB, and tetS) were not detected by quantitative PCR in many samples. In contrast, sul1 and sul2 (coding for sulfonamide resistance) were present at relatively hig...

This study was conducted in order to assess the antimicrobial resistance patterns of E. coli isolated from young animals affected between 1980 and 2016. The selected isolates for this study (n=175) carried stx1/stx2 genes and the most prevalent type of pathogenic E. coli found belonged to serogroup O101, antigen (K99)-F41 positive. All STEC-positive isolates were tested for susceptibility to 11...

Determinants of tetracycline resistance have been cloned from two different tetracycline-producing industrial strains of Streptomyces into Streptomyces lividans using the plasmid vector pUT206. Three plasmids, pUT250 and pUT260 with a 9.5 and a 7.5 kb insert respectively of Streptomyces rimosus DNA, and pUT270 with a 14.0 kb insert of Streptomyces aureofaciens DNA, conferring resistance to tetr...

The phylogenetic relationships of 17 Bacillus strains isolated from plants and soil were determined from partial sequences of genes encoding 16S rRNA, gyraseA (gyrA) and the cheA histidine kinase. Five strains were closely related to Bacillus subtilis subsp. subtilis, three strains were more closely related to B. subtilis subsp. spizizeni and two strains were identified as B. mojavensis. The re...

Approximately 39.9% (63/158) of beef samples collected from retail markets in Hanoi from January to June 2009 were Salmonella-positive. Nine Salmonella serovars, Anatum (28.6%), Rissen (25.4%), Weltevreden (12.7%), Typhimurium (7.9%), Derby (7.9%), Lexington (7.9%), Dublin (4.6%), Newport (3.2%) and London (1.8%), were identified. Thirty-seven (58.7%) of the 63 Salmonella isolates were resistan...