Sperm acrosomes contain hyaluronidase, the amount varying, respectively, from ram, rabbit, bull, human, boar, rat, and stallion sperm to rooster sperm that do not possess this enzyme. Hyaluronidase was partially purified from bull sperm acrosomal extracts by DEAE-cellulose chromatography. Bull sperm acrosomal hyaluronidase has an optimum pH of 3.75 and is not effected by freezing and thawing or...

BACKGROUND Sperm quality may degrade during transit through the male reproductive tract in some individuals. In this setting surgically retrieved testicular sperm may outperform ejaculated samples for use with in vitro fertilization (IVF) and intracytoplasmic sperm injection (IVF-ICSI). We sought to describe one center's experience with the use of fresh testicular sperm after prior failed IVF-I...

background: extracted sperm from the testis have poor motility. moreover, their motility changes during their journey through epidydimis. meanwhile, they face high concentration of l-carnitin. in addition, lactate dehydrogenase c4 (ldh-c4) gene disorders has been shown to cause impaired sperm motility, leading to infertility in male mice. the aim of this study was to evaluate sperm motility and...

Cryopreservation of immature testicular tissues is essential for increasing the possibilities of offspring generation by testicular xenografting for agricultural or medical purposes. However, successful production of offspring from the sperm involved has never been reported previously. In the present study, therefore, using intracytoplasmic sperm injection (ICSI), we examined whether xenogeneic...

PURPOSE To assess the impact of sperm retrieval on the gonadal function of rats with impaired spermatogenesis by comparing testicular sperm extraction (TESE) to aspiration (TESA). The efficacy of these procedures to sperm obtainment was also compared. MATERIALS AND METHODS A pilot study showed impaired spermatogenesis, but normal testosterone (T) production after a bilateral orchidopexy appli...

The effect of in-vitro culture on the motility and morphology of fresh and frozen-thawed human testicular spermatozoa obtained from obstructive azoospermic patients and on the motility of testicular spermatozoa obtained from non-obstructive azoospermic patients was evaluated. The outcome of intracytoplasmic sperm injection (ICSI) with fresh and frozen-thawed human testicular spermatozoa was stu...

We are thankful to Dr. Drobnis for her insights and balanced comments with regard to our article titled “comparison of sperm retrieval and reproductive outcome in azoospermic men with testicular failure and obstructive azoospermia treated for infertility”.1 Our main objective was to offer firm information that could be used as a counseling tool by doctors treating patients with azoospermia‐rela...

Men with congenital absence or bilateral partial aplasia of vas deferens, or those with failed or surgically unreconstructable obstructions or azospermic men with testicular failure or genetic cause can now be treated by using sperm retrieval techniques in conjunction with in vitro fertilization/intra cytoplasmic sperm injection (ICSI/ IVF). These techniques are: Microsurgical epididymal sperm ...

BACKGROUND Sperm DNA damage (fragmentation) is a recently discovered cause of male infertility for which no efficient treatment has yet been found. Previous findings have suggested that clinically relevant sperm DNA damage may occur at the post-testicular level. This study was undertaken to assess the clinical usefulness of ICSI with testicular spermatozoa in this indication. METHODS The perc...

Advances of the last two decadesin the treatment of azoospermic men enabled recovery of testicular spermatozoa and fertilization of the oocytes with ICSI. Since the introduction of the retrieval of testicular sperm by tissue extraction [1,2], ICSI with testicular sperm has become a routine procedure initially for patients with obstructive azoospermia (OA) [1,2], and later for non-obstructive az...