The mouse anterior pituitary tumor cell line, AtT-20, targets secretory proteins into two distinct intracellular pathways. When the DNA that encodes trypsinogen is introduced into AtT-20 cells, the protein is sorted into the regulated secretory pathway as efficiently as the endogenous peptide hormone ACTH. In this study we have used double-label immunoelectron microscopy to demonstrate that try...

The prolactin releasing peptide (PrRP) is involved in regulating food intake and body weight homeostasis, but molecular details on the activation of the PrRP receptor remain unclear. C-terminal segments of PrRP with 20 (PrRP20) and 13 (PrRP8-20) amino acids, respectively, have been suggested to be fully active. The data presented herein indicate this is true for the wildtype receptor only; a 5-...

Human islet amyloid polypeptide (hIAPP) accumulates as pancreatic amyloid in type 2 diabetes and readily forms fibrils in vitro. Investigations into the mechanism of hIAPP fibril formation have focused largely on residues 20 to 29, which are considered to comprise a primary amyloidogenic domain. In rodents, proline substitutions within this region and the subsequent beta-sheet disruption, preve...

The three-dimensional structure of a synthetic peptide corresponding to the N-terminal membrane anchor domain (residues 1-25) of prostaglandin I(2) synthase (also known as cytochrome P450 8A1), an eicosanoid-synthesizing microsomal cytochrome P450, has been determined by two-dimensional (1)H NMR spectroscopy in trifluoroethanol and dodecylphosphocholine which mimic the hydrophobic membrane envi...

Background Hypertension is the second most common cause of maternal death in world. Predicting occurrence preeclampsia (PE) complications needed to optimize management. Brain natriuretic peptide (BNP) a polypeptide, secreted by cardiac ventricular myocytes. Research linking N-Terminal Pro B-Type Natriuretic Peptide (NT-proBNP) levels with has never been conducted Indonesia. The aim study evalua...

      Kawasaki disease (KD) is characterized as the leading cause of acquired cardiac disease in children. Accurate and timely diagnosis of KD is of high importance for preventing its cardiac complications. However, diagnosis merely based on clinical findings has a number of challenges and, limitations. Therefore, researchers are investigating to find more object...

We have previously shown that the peroxisomal targeting signal in firefly luciferase consists of the COOH-terminal three amino acids of the protein, serine-lysine-leucine (Gould, S.J., G.A. Keller, N. Hosken, J. Wilkinson, and S. Subramani, 1989. J. Cell Biol. 108:1657-1664). Antibodies were raised against a synthetic peptide that contained this tripeptide at its COOH terminus. Immunofluorescen...

We tested the capacity of antibodies RB733, RB734, RB766, RB767, RB768, RB769, RB770, RB771, RB772 and RB773 to recognize by ELISA three different molecular forms human cardiac troponin T isoform 6 (cTnT): an N-terminal peptide (Nter-cTnT), full-length cTnT protein (FL-cTnT) I-T-C complex (ITC-complex). Our results show that RB734 specifically recognizes region as well alone ITC-complex. or in ...

We have found through ex vivo and in vivo angiogenesis models that the adrenomedullin gene-related peptide, proadrenomedullin NH2-terminal 20 peptide (PAMP), exhibits a potent angiogenic potential at femtomolar concentrations, whereas classic angiogenic factors such as vascular endothelial growth factor and adrenomedullin mediate a comparable effect at nanomolar concentrations. We found that hu...