Protoplasts isolated from tobacco leaves were inoculated with cucumber mosaic virus (CMV) using a method slightly modified from that developed for inoculating tobacco mosaic virus. Multiplication of CMV was demonstrated by staining the protoplasm with fluorescent CMV-antibody, by the electron microscopy of ultrathin sections, and by assaying the infectivity in protoplasts. Seventy to 90% of pro...

Tobacco mosaic virus (TMV) RN.4 and TMV coat protein were reconstitut,ed in the presence of an excess of host RNA or turnip yellow mosaic virus (TYMV) RNA. TMV protein and its homologous RNA recognized each other specifically in the presence of an excess of host RNA, but when TMV RNA and TYMV RNA were mixed with protein, both RNAs were encapsidated. Indeed, at pH 6, TYMV RNA was recognized by T...

The past fifty years important advances have been made in the field of grapevine virus research, including characterization of pathogens and control measurements. Still the occurrence of Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Tomato black ring virus (TBRV), Grapevine chrome mosaic virus (GCMV), Alfalfa mosaic virus (AMV), Grapevine Bulgarian latent virus (GBLV), Grapevine f...

turnip mosaic virus (tumv), cauliflower mosaic virus (camv) and beet western yellow virus (bwyv) are considered as the most important viruses of canola, worldwide. in order to determine the distribution of these viruses in golestan province, samples from three important canola growing areas of the province were collected during 2008 and 2009. the randomly collected samples were tested for the p...

Artificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically downregulate RNAs that contain complementary sequences. Transgenic plants expressing high levels of one or more amiRNAs targeting particular sequences in the genomes of some RNA viruses have shown specific resistance to the corresponding virus. This is the case of th...

two hundred and thirty one leaf samples of virus infected and mosaic and as well dwarf mosaic symptoms showing maize plants from various corn fields in tehran province were collected during the growing season 2003. by performing serological tests of das-elisa, diba and tpia, using scmv, mdmv, srmv and jgmv antisera, only scmv was detected in samples, with all samples reacting strongly to the sc...

Background and Aims: Celery mosaic virus (CeMV) is one of the causal agents of viral diseases in celery (Apium graveolens). CeMV is a member of the Potyvirus genus in Potyviridae family. The virus is naturally transmitted by aphids in a non-persistent manner. During growing season 2006-2007 viral disease symptoms were observed in celery fields grown in Tehran Province (Bage daneshkade, Mohamads...

1. Denatured tobacco mosaic virus has a number of SH groups corresponding to its total sulfur content of 0.2 per cent. The SH groups were estimated by titration with ferricyanide, tetrathionate, and p-chloromercuribenzoate in guanidine hydrochloride solution and by reduction of the uric acid reagent in urea solution. 2. The SH groups of tobacco mosaic virus or their precursors can be abolished ...

Genomic components of the begomovirus causing yellow mosaic disease (YMD) in soybean in Delhi were cloned, sequenced and infectivity proved. Nucleotide sequence analysis of the virus isolate revealed more than 89% identity with Mungbean yellow mosaic India virus (MYMIV) and therefore it is designated as soybean isolate of MYMIV (MYMIV-[Sb]). Total nucleotide and predicted amino acid sequence an...