Fructus Arctii is used as a traditional herbal medicine to treat inflammatory diseases in oriental countries. This study aimed to investigate effect of F. Arctii extract on intestinal barrier function in human intestinal epithelial Caco-2 cells and to reveal the active component of F. Arctii. We measured transepithelial electrical resistance (TEER) value (as an index of barrier function) and ov...

Rabbit aortic endothelial cells (RAECs) were grown on micropore filters in a device that allowed in situ determination of transendothelial electrical resistance (TEER). Incubation of confluent RAEC monolayers with 2 ng.ml-1 of bacterial LPS for 3 h did not change the protein content or the number of cells on the filters, but resulted in a marked decline in TEER (from 14.1 +/- 0.9 to 5.1 +/- 0.6...

Endothelial p120-catenin (p120) maintains the level of vascular endothelial cadherin (VE-Cad) by inhibiting VE-Cad endocytosis. Loss of p120 results in a decrease in VE-Cad levels, leading to the formation of monolayers with decreased barrier function (as assessed by transendothelial electrical resistance [TEER]), whereas overexpression of p120 increases VE-Cad levels and promotes a more restri...

Listeria monocytogenes is a food and soil-borne pathogen that secretes a pore-forming toxin listeriolysin O (LLO) as its major virulence factor. We tested the effects of LLO on an intestinal epithelial cell line Caco-2 and compared them to an unrelated pore-forming toxin equinatoxin II (EqtII). Results showed that apical application of both toxins causes a significant drop in transepithelial el...

Tricuspid regurgitation (TR) has been associated with worse outcomes after transcatheter edge-to-edge repair of the mitral valve (TEER). There are limited data about impact TR on health status TEER. Health was assessed Kansas City Cardiomyopathy Questionnaire-overall summary score (KCCQ-OS) in patients enrolled STS/ACC TVT Registry who received isolated TEER between Jan 2019-Jun 2021. Multivari...

Abstract Background Right ventricular dysfunction (RVD) is an important predictor for outcome in patients undergoing transcatheter mitral or tricuspid valve edge-to-edge repair (M/T-TEER). Due to the unique anatomy and contraction pattern of RV, three-dimensional echocardiography (3DE) has emerged as a valuable tool assessment RV function. While 3DE data showed reverse remodeling (RVRR) followi...

The aim of this investigation was to design and develop nanoemulsions as novel delivery systems for rapamycin. Phase behavior of quaternary systems composed of Traicetin (as oil), various surfactants and co-surfactants and water at different surfactant/co-surfactant weight ratios were investigated by the construction of phase diagrams. Formulations were taken from the o/w NE region of the phase...

Concomitant mitral valve surgery during septal myectomy for hypertrophic cardiomyopathy (HCM) is performed in <10% of patients and associated with increased mortality. The objective this study to evaluate the feasibility, safety, outcomes transcatheter edge-to-edge repair (TEER) HCM regurgitation (MR) after myectomy. Consecutive cases at a single center from 01/2019 06/2022 (n=90) were retrospe...

As the New Zealand population ages, incidence of heart failure and valvular disease increases. The Cardiovascular Outcomes Assessment MitraClip Percutaneous Therapy for Heart Failure Patients with Functional Mitral Regurgitation (COAPT) trial showed decreased rates hospitalisation all-cause mortality transcatheter edge-to-edge repair (TEER) clinically significant secondary mitral regurgitation ...

Abstract Background Guideline-directed medical therapy (GDMT) optimization is mandatory before transcatheter edge-to-edge mitral valve repair (M-TEER) in patients with secondary regurgitation (SMR) and heart failure reduced ejection fraction (HFrEF). However, the effect of M-TEER on GDMT unknown. Objective To evaluate frequency prognostic implications predictors after SMR HFrEF. Methods This a ...