نتایج جستجو برای: taq polymerase

تعداد نتایج: 128420  

Journal: :FEMS microbiology letters 1993
M M Willcocks J G Silcock M J Carter

We have developed a polymerase chain reaction for the detection of Norwalk virus using the published sequence of the virus RNA dependent RNA polymerase gene and have used this to clone and sequence this region of a virus from a UK outbreak. We have applied this method to a panel of UK Norwalk-like viruses using both Tet-z and Taq DNA polymerases and found that amplification produces a multiplic...

Alireza Rafiei, Fatemeh Moradian, Mohammad Alavi, Mohammad Bagher Hashemi-Sotehoh, Reza Valadan, Touraj Farazmandfar,

Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the T...

2015
Lilja Björk Jónsdóttir

................................................................................................................................... iii Útdráttur ................................................................................................................................... v Acknowledgement ........................................................................................................

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1989
P Keohavong W G Thilly

Denaturing gradient gel electrophoresis (DGGE) was used to separate and isolate the products of DNA amplification by polymerase chain reaction (PCR). The strategy permitted direct enumeration and identification of point mutations created by T4, modified T7, Klenow fragment of polymerase I, and Thermus aquaticus (Taq) DNA polymerases. Incorrectly synthesized sequences were separated from the wil...

2014
Peter McInerney Paul Adams Masood Z. Hadi

As larger-scale cloning projects become more prevalent, there is an increasing need for comparisons among high fidelity DNA polymerases used for PCR amplification. All polymerases marketed for PCR applications are tested for fidelity properties (i.e., error rate determination) by vendors, and numerous literature reports have addressed PCR enzyme fidelity. Nonetheless, it is often difficult to m...

2012
Jake Juyoung Guag Michael L. Grantham

Title of Document: RESIDUAL DNA IN COMMERCIAL TAQ DNA POLYMERASE AS A SOURCE OF INTERFERENCE WITH IMMUNO-PCR ASSAY Jake Juyoung Guag, MPH, 2012 Directed By: Professor and Director, Dr. Donald K. Milton, Maryland Institute for Applied Environmental Health Polymerase Chain Reaction (PCR) was developed for a broad range of purposes. As part of developing a very sensitive Immuno-quantitative PCR (i...

2000
Ming-Yi Zhou Celso E. Gomez-Sanchez

TA cloning is one of the simplest and most efficient methods for the cloning of PCR products. The procedure exploits the terminal transferase activity of certain thermophilic DNA polymerases, including Thermus aquaticus (Taq) polymerase. Taq polymerase has non-template dependent activity which preferentially adds a single adenosine to the 3'-ends of a double stranded DNA molecule, and thus most...

Journal: :Journal of biochemistry and molecular biology 2002
Fátima Martel Dirk Gründemann Edgar Schömig

Discrimination between the amplification of mRNA and contaminating genomic DNA is a common problem when performing a reverse transcriptase-polymerase chain reaction (RT-PCR). Even after treatment of the samples with DNAse, it is possible that negative controls (samples in which no reverse transcriptase was added) will give positive results. This indicates that there was amplification of DNA, wh...

Journal: :Journal of clinical microbiology 2011
Hideki Niimi Masashi Mori Homare Tabata Hiroshi Minami Tomohiro Ueno Shirou Hayashi Isao Kitajima

To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using euka...

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