The step of maturation (around 60 days) appeared less active as compared to the first step of pre-fermentation (around 90 days) since the values of temperature, recorded during the second step of maturation, are found generally less important than those recorded during the first step. A similar tendency of C/N ratio values decrease is generally observed in the two piles of wastes during the two...

Coliform bacteria were isolated from raw sewage and sewage effluent-receiving waters and tested for their antibiotic susceptibility patterns and their ability to transfer antibiotic resistance to Escherichia coli K-12 C600. An environmental isolate of E. coli (MA527) capable of transferring antibiotic resistance to C600 was mated, both in vitro and in situ, with an antibiotic-sensitive E. coli ...

Objective: To evaluate the quality of seeds tomato fruits (Solanum lycopersicum Mill.) produced in high temperture (HT) during different phases development. Design/Methodology/Approach: Seeds Moneymaker variety were planted a ventilated greenhouse (control treatment, CT) with maximum mean temperature (MMT) 31.5 °C. A second artificial heating (MMT 36.5 ºC) was used for HT treatments. When anthe...

background: recently, the use of t7 rna polymerase instead of other viral and cellular promoters is increasing due to high efficacy of transcription in the cell cytoplasm by this polymerase. in order to translate the transcripts produced by t7 rna polymerase in mammalian cell lines, it is necessary to include internal ribosome entry site (ires) sequences. in addition, if sequence of poly a sign...

A series of nickel complexes containing N5-pentadentate ligands with different amine-to-pyridine ratios were studied for electrochemical H2 production in neutral water and the one with a diamine-tripyridine ligand displays a TON of up to 308,000 over 60 h electrolysis at -1.25 V vs. SHE, with a Faradaic efficiency of ∼91%.

Phage T7 helicase unwinds double-stranded DNA (dsDNA) by encircling one strand while excluding the complementary strand from its central channel. When T7 helicase translocates on single-stranded DNA (ssDNA), it has kilobase processivity; yet, it is unable to processively unwind linear dsDNA, even 60 base-pairs long. Particularly, the GC-rich dsDNAs are unwound with lower amplitudes under single...

Phytoremediation is the technique based on the use of plants to remediate sites contaminated with organic and inorganic pollutants. This technology is rapidly growing as is helpful to growing industries. Colocasia esculenta is a tropical plant grown primarily for its edible corm and the root vegetables. It has many names like Taro and Eddoe. It is a wide spread emergent aquatic plant, generally...

producing biogas is the most common method of energy generation in municipal and industrial wastewater treatment plants in the world. methane production rate depends on degradation rate of organic pollutants in wastewater, temperature and treatment process. typical parameters for assessment of organic wastewater such as bod or cod indicate the potential to produce methane. there is no accurate ...

An elastic DNA molecular mechanics model is used to compare DNA structures and packing thermodynamics in two bacteriophage systems, T7 and phi29. A discrete packing protocol allows for multiple molecular dynamics simulations of the entire packing event. In T7, the DNA is coaxially spooled around the cylindrical core protein, whereas the phi29 system, which lacks a core protein, organizes the DN...