The interactions of crops with root-colonizing endophytic microorganisms are highly relevant to agriculture, because endophytes can modify plant resistance pests and increase crop yields. We investigated the between host Zea mays fungus Trichoderma virens at 5 days postinoculation grown in a hydroponic system. Wild-type T. two knockout mutants, deletion genes tv2og1 or vir4 involved specialized...

Ustilaginoidea virens, the causal agent of rice false smut, is one of the most devastating grain diseases that causes loss of yield in most rice-growing areas worldwide. In this study, we performed a dsRNA screen to isolate mycoviruses from 35 U. virens strains. The results revealed that 34 of the tested isolates were infected by various dsRNA elements, displaying highly viral diversity and mix...

in the present study, the diversity of trichoderma spp. was investigated in alkaline pistachio soils at different geographic area in kerman province, iran. a total of 161 trichoderma isolates were obtained and identified at the species level by analysis of their morphological characters and sequence analysis of their internal transcribed spacer regions of the rrna cluster and tef1 genes as phyl...

Seven different strains of Trichoderma are isolated from wilt infected leguminous crops of a Madhya Pradesh state and tested for their antagonistic activity against Fusarium (soil borne pathogen) which is expressed as a zone of inhibition in the culture plates. The seven strains are identified as Trichoderma viride, T. harzianum, T. asperellum, T. koningii, T. atroviride, T. longibrachiatum, an...

Empty Fruit Bunches (EFB) and Palm Oil Mill Effluent (POME) are two main wastes from oil palm industries which contain rich lignocellulose. Degradation of EFB and POME by microorganisms will produce hydrolytic enzyme which will degrade cellulose and hemicellulose during composting process. However, normal composting takes about four to six months to reach maturity. Hence, application of fungi i...

Rice false smut caused by Ustilaginoidea virens is one of the most important diseases of rice worldwide. Although its genome has been sequenced, to date there is no report on targeted gene deletion in U. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. In this study, we attempted to generate knockout mutants of the ortholog o...

BACKGROUND Ancylostoma species have demanded attention due to their zoonotic potential. The use of anthelmintics is the usual method to prevent environmental contamination by Ancylostoma eggs and larvae. Nematophagous fungi have been widely used in their biological control due to the fungus ability to capture and digest free nematode forms. AIMS The aim of this study was to evaluate the effec...

Twenty-eight potential biocontrol organisms were tested for efficacy against Rhizoctonia solani on potato in a series of greenhouse trials. Organisms tested consisted of field isolates of Paenibacillus polymyxa, Pseudomonas fluorescens, Penicillium sp., Trichoderma sp., and Rhizoctonia zeae; known biocontrol isolates including Laetisaria arvalis, Verticillium biguttatum, Cladorrhinum foecundiss...

Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus Gliocladium virens and to transform them to benomyl resistance with a Neurospora crassa beta-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative an...