efficient use of agricultural wastes due to their recycling and possible production of cost effective materials, have economic and ecological advantages. a biological method used for degrading agricultural wastes is a new method for improving the digestibility of these materials and favoring the ease of degradation by other microorganisms. this research was carried out to study the possible bio...

BACKGROUND Global climate change and fossil fuels limitations have boosted the demand for robust and efficient microbial factories for the manufacturing of bio-based products from renewable feedstocks. In this regard, efforts have been done to enhance the enzyme-secreting ability of lignocellulose-degrading fungi, aiming to improve protein yields while taking advantage of their ability to use l...

BACKGROUND The induction of cellulase production by insoluble carbon source cellulose was a common and efficient strategy, but has some drawbacks, such as difficult fermentation operation, substantial cellulase loss, long fermentation time, and high energy-consumption, resulting in high cost of cellulase production in industry. These drawbacks can be overcome if soluble carbon sources are utili...

The adsorption parameter, A,, (maximum protein adsorbed/g substrate) and KA (adsorption equilibrium constant) of Trichoderma reesei C-5 cellulases were 58.8 mg protein/g cellulose and 11.6 x 104 Z/m01 respectively. The activation energies for the adsorption rate constants of cellobiobydrolases and endoglucanases of C-5 were 30% and 11% lower respectively than that in the parent T. reesei QM9414...

BACKGROUND Trichoderma reesei is the principal producer of cellulolytic enzymes. Because of the strong influence on the enzyme production, the morphology of the filamentous fungi is a key parameter for process optimization. For cost-effective production of cellulolytic enzymes, the cultivation of T. reesei is performed on lignocellulosic waste streams. These insoluble substrates prevent the app...

Trichoderma reesei is widely applied as the major industrial fungus for production of cellulases used conversion lignocellulosic biomass to biofuels and other biobased products. The protein secretion pathway vital cellulase secretion, but few reports are related role vacuole in production. Here, we identified a novel vacuolar serine protease gene spt1 investigated ability T. secrete by disrupti...

Enzymes are proteins that catalyse chemical reactions by lowering the activation energy needed and thus speed up the reaction itself. The advantage of enzymes is that they can be applied under mild reaction conditions and that they exhibit high substrate specificity, stereoselectivity and less side product formation than conventional chemical reactions, making biotechnological processes often m...

Although it is well known that low nitrogen stimulates lipid accumulation, especially for algae and some oleaginous yeast, few studies have been conducted in fungal species, especially on the impact of different nitrogen deficiency strategies. In this study, we use two promising consolidated bioprocessing (CBP) candidates to examine the impact of two nitrogen deficiency strategies on lipid prod...

Abstract. In our previous studies, we filtered out fungus (Trichoderma reesei) to have the best ability transform corn straw into a humic-acid-like substance through laboratory incubation experiments. order further verify former findings, set up 360 d field experiment that included three treatments applied under equal carbon (C) mass: (i) returned (CS), (ii) fermented treated with Trichoderma r...

The filamentous fungus, Trichoderma viride, secretes large amounts of several cellulolytic enzymes (1). We report here the nucleotide sequence of the gene coding for exo-cellobiohydrolase (CBH), which accounts for more than 65% of the total cellulase content. This gene was isolated from a T. viride genomic library using a 30mer oligonucleotide based on the sequence of the reported CBHI gene of ...