نتایج جستجو برای: sybr green i

تعداد نتایج: 1167642  

Journal: :Memorias do Instituto Oswaldo Cruz 2003
Claudia C Rubiano Moises Wasserman

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

Journal: :Oncology reports 2009
Guan-Li Huang Xiao-Hua Zhang Gui-Long Guo Ka-Te Huang Kai-Yan Yang Xian Shen Jie You Xiao-Qu Hu

Growing evidence suggests microRNAs (miRNAs) have an important role in tumorigenesis. MicroRNA-21 (miR-21) is up-regulated in many malignant tumors, including breast cancer. Its association with clinicopathologic features and expression of PTEN (phosphatase and tensin homolog deleted on chromosome 10), one of its target genes, in breast cancer has not been reported systematically. To further de...

2014
Zahra Vali Abbasali Raz Hanieh Bokharaei Mohammad Nabavi Mohammad Hassan Bemanian Mina Sharifi Yazdi Navid Dinparast Djadid

BACKGROUND Asthma is caused by the combination of different factors. Current concepts of asthma pathogenesis emphasize on gene-environment interactions. Mega-genome scanning projects revealed that different Single Nucleotide Polymorphisms (SNPs) are related to asthma susceptibility. rs7216389-T is one of them that is related to childhood asthma and its effect on childhood asthma severity has be...

Journal: :iran agricultural research 2016
l. lakzadeh s. hosseinzadeh s.s. shekarforoush m. fazeli

abstract- today, the authenticity of meat products with less costly and desirable species has increased. therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. in this study, real time pcr assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. specific pri...

Journal: :jundishapur journal of microbiology 0
mozhdeh sultani virology department, school of public health, tehran university of medical sciences, tehran, ir iran talat mokhtari azad virology department, school of public health, tehran university of medical sciences, tehran, ir iran mohammadreza eshragian virology department, school of public health, tehran university of medical sciences, tehran, ir iran azadeh shadab virology department, school of public health, tehran university of medical sciences, tehran, ir iran maryam naseri virology department, school of public health, tehran university of medical sciences, tehran, ir iran owrang eilami department of infectious disease, yasuj university of medical sciences, yasuj, ir iran

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

2013
Rocio Sanjuan-Jimenez Juan D. Colmenero Pilar Bermúdez Antonio Alonso Pilar Morata

Some sites of extrapulmonary tuberculosis and focal complications of brucellosis are very difficult to differentiate clinically, radiologically, and even histopathologically. Conventional microbiological methods for the diagnosis of extrapulmonary tuberculosis and complicated brucellosis not only lack adequate sensitivity, they are also time consuming, which could lead to an unfavourable progno...

Journal: :Acta histochemica 2005
Candida Briggs Mark Jones

Using fluorescence microscopy, we explored the ability of cultured immune cells to take up aqueous SYBR Green I (SGI). SYBR Green I, a highly sensitive fluorescent nucleic acid stain, which preferentially binds to dsDNA over ssDNA or RNA with little background fluorescence from unbound molecules. A time course study over 2h using final dilutions of SGI of 1:10,000 and 1:100,000 at 22 and 37 deg...

Journal: :Archives of Iranian medicine 2011
Kiana Shahzamani Farzaneh Sabahi Shahin Merat Majid Sadeghizadeh Hamed Esmail Lashkarian Houri Rezvan Siamak Mirab Samiee Mohsen Karimi Arzanani Hossain Jabbari Reza Malekzadeh

BACKGROUND We intend to design and validate a low-cost assay for the detection of hepatitis C virus (HCV) RNA using rapid-cycle RT-PCR. The procedure is performed in a closed system with little risk of contamination allowing PCR and product identification to be performed within one or two hours. METHODS A SYBR Green-based real-time RT-PCR for rapid detection of HCV. Amplicon synthesis was mon...

Journal: :Clinical chemistry 2006
Mark G Herrmann Jacob D Durtschi L Kathryn Bromley Carl T Wittwer Karl V Voelkerding

BACKGROUND DNA melting analysis for genotyping and mutation scanning of PCR products by use of high-resolution instruments with special "saturation" dyes has recently been reported. The comparative performance of other instruments and dyes has not been evaluated. METHODS A 110-bp fragment of the beta-globin gene including the sickle cell anemia locus (A17T) was amplified by PCR in the presenc...

2014
Tsegalem Abera Ardhanary Thangavelu Navamani Daniel Joy Chandran Angamuthu Raja

BACKGROUND Peste des petits ruminants (PPR) is an economically important disease of small ruminants such as sheep and goats. The disease is characterized by severe pyrexia, oculo-nasal discharge, pneumonia, necrosis and ulceration of the mucous membrane and inflammation of the gastro-intestinal tract leading to severe diarrhea. A SYBR Green I based real time RT-PCR targeting the N gene of PPRV ...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید