Recombinant human deoxyribonuclease (rhDNase) has been demonstrated to reduce the viscosity of purulent cystic fibrosis (CF) respiratory mucus, to improve pulmonary function and to reduce the risk of respiratory tract infectious exacerbations, but its effect on mucus transportability has not so far been investigated. The dose-dependent effect of rhDNase was analysed in vitro on mucus transport ...

The mucus that protects the surface of the gastrointestinal tract is rich in specialized O-glycoproteins called mucins, but little is known about other mucus proteins or their variability along the gastrointestinal tract. To ensure that only mucus was analyzed, we combined collection from explant tissues mounted in perfusion chambers, liquid sample preparation, single-shot mass spectrometry, an...

Nanoparticles larger than the reported mesh-pore size range (10-200 nm) in mucus have been thought to be much too large to undergo rapid diffusional transport through mucus barriers. However, large nanoparticles are preferred for higher drug encapsulation efficiency and the ability to provide sustained delivery of a wider array of drugs. We used high-speed multiple-particle tracking to quantify...

background: childhood masturbation (cm) is considered a variant of normal sexual behavior; however, it is commonly misdiagnosed as epilepsy and movement disorders. as the first study from iran, we analyzed a large population of infants and children with cm in a case-control study and evaluated the value of mucus in urine analysis as an alternative diagnostic tool for cm. methods: a total of 623...

AIM To investigate colonic mucus thickness in vivo in health and during experimental inflammatory bowel disease. METHODS Colitis was induced with 5% DSS in drinking water for 8 days prior to experiment, when the descending colonic mucosa of anesthetized rats was studied using intravital microscopy. Mucus thickness was measured with micropipettes attached to a micromanipulator. To assess the c...

Overview Corals have been shown to harbor unique microbial communities associated with their surface mucopolysaccharide layer, which is hypothesized to play an important role in defense against invading pathogens (Ritchie and Smith, 2004). In this study, bacterial associates of Montastraea faveolata surface mucus were compared between the healthy, disease-free reefs of the Flower Garden Banks N...

Ulcerative colitis (UC) is the result of an inappropriate colonic inflammatory response triggered by environmental and genetic factors. We have recently shown that mucus from UC patients has a decreased phosphatidylcholine (PC) content, while clinical trials revealed that therapeutic addition of PC to the colonic mucus alleviated the inflammatory activity. The mechanisms behind this are still u...

for the study of the physiology of gastric mucus secretion, numerous agents have been investigated in this laboratory (9, 10, 11). Gentle massage, aqueous ether (saturated), 5 per cent aqueous clove oil emulsion, 50 per cent ethyl alcohol, dis tilled water, isotonic and hypertonic NaCl, and @ to 5 per cent aqueous eugenol emulsions were all found to induce desquamation of the surface epithelium...

Food and probiotic bacteria, in particular lactic acid bacteria, are ingested in large amounts by humans and are part of the transient microbiota which is increasingly considered to be able to impact the resident microbiota and thus possibly the host health. The lactic acid bacterium Lactococcus lactis is extensively used in starter cultures to produce dairy fermented food. Also because of a ge...

Capillary instability of a two-layer liquid film lining rigid tube is studied computationally as model for plug formation and closure human airways. The consists serous layer, also called the periciliary at inner side mucus layer outer side. Together, they form airway surface wall surrounding an air core. Liquid occurs due to Plateau–Rayleigh when thickness exceeds critical value. Numerical sim...