Isothiocyanates (ITCs) from cruciferous vegetables have been shown to be effective in blocking initiation as well as progression of a range of chemically-induced tumors in animal models. In this study, sulforaphane, the most extensively studied ITC, was found to suppress the growth of T24 bladder cancer cells in vitro in a dose-dependent manner. Sulforaphane inhibited the proliferation of T24 c...

Sulforaphane increases the expression of dihydrotestosterone (DHT)-degrading enzyme, 3α-hydroxysteroid dehydrogenase (3α-HSD) in liver, which accelerates DHT degradation, thereby inhibiting hair loss animal model. In this study, we elucidated its underlying mechanism and demonstrated that sulforaphane has inhibitory functions RAW264.7 macrophage cells Hepa1c1c7 at cellular gene levels. The leve...

Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)butane], a naturally occurring isothiocyanate derived from cruciferous vegetables, is a highly potent inducer of phase 2 cytoprotective enzymes and can protect against electrophiles including carcinogens, oxidative stress, and inflammation. The mechanism of action of sulforaphane is believed to involve modifications of critical cysteine residues ...

Advanced androgen-independent prostate cancer (AIPC) is an aggressive malignancy with a poor prognosis. Apoptosis-resistant cancer stem cells (CSCs) have been identified in AIPC and are not eliminated by current therapeutics. Novel therapeutic options, which are currently being evaluated in patient studies, include TRAIL and the broccoli-derived isothiocyanate sulforaphane. Although neither age...

Oxidative stress is an important molecular mechanism of astrocyte injury and death following ischemia/reperfusion and may be an effective target of intervention. One therapeutic strategy for detoxifying the many different reactive oxygen and nitrogen species that are produced under these conditions is induction of the Phase II gene response by the use of chemicals or conditions that promote the...

Sulforaphane (1-isothiocyanate-4-methyl sulfonyl butane) is a plant extract (obtained from cruciferous vegetables, such as broccoli and cabbage) and is known to exert anticancer, antioxidant and anti-inflammatory effects. It stimulates the generation of human or animal cells, which is beneficial to the body. The aim of the current study was to determine whether sulforaphane protects against lip...

Epidermal squamous cell carcinoma (SCC) is among the most common cancers. SCC can be treated by surgical excision, but recurrence of therapy-resistant disease is a major problem. We recently showed that YAP1, the Hippo signaling transcription adaptor protein, and ∆Np63α, a key epidermal stem cell survival protein, form a complex to drive epidermal cancer stem cell survival. In the present study...

The effects of storage conditions and cooking methods on chlorophyll, glucosinolate (GSL), sulforaphane content in broccoli florets were investigated this study. For the experiment, fresh stored for 2, 4, 6 days at 10, 0 °C with or without 1-methylcyclopropene (1-MCP) treatment. cooked 1, 3, 5, 7, 9 min under three methods, namely, steaming, microwaving, boiling. Results showed that contents al...

sulforaphane (sfn) is an isothiocyanate which is produced through glucoraphanin hydrolysis via myrosinase activity. in the present study, sfn content and peroxidase activity has been assessed in treated lepidium draba seedlings with different concentrations of iron and copper during 8 and 16 hours treatment. the results showed that the sfn content drastically elevated at treatment with 1 mg/l f...

Two population-based, case-control studies have documented reduced risk of prostate cancer in men who consume cruciferous vegetables. Cruciferae contain high levels of the isothiocyanate sulforaphane. Sulforaphane is known to bolster the defenses of cells against carcinogens through up-regulation of enzymes of carcinogen defense (phase 2 enzymes). Prostate cancer is characterized by an early an...