Feruloyl esterases, a subclass of the carboxylic acid esterases (EC 3.1.1.1), are able to hydrolyse the ester bond between the hydroxycinnamic acids and sugars present in the plant cell wall. The enzymes have been classified as type A or type B, based on their substrate specificity for aromatic moieties. We show that Neurospora crassa has the ability to produce multiple ferulic acid esterase ac...

The isolation of component sugars from biomass represents an important step in the bioprocessing of sustainable feedstocks such as sugar beet pulp. Centrifugal partition chromatography (CPC) is used here, as an alternative to multiple resin chromatography steps, to fractionate component monosaccharides from crude hydrolysed sugar beet pulp pectin. CPC separation of samples, prepared in the stat...

twenty four multiparous holstein cows in their early stage of lactation with a mean of 59±20 dim and a mean of 615±40.5 kg of bw were employed in a complete randomized block design to evaluate the effects of different levels of sugar beet pulp in a partial replacement for alfalfa, the only source of forage in their previous diet (treat 1, 0% beet pulp and 35% alfalfa, treat 2 ,6% beet pulp and ...

Sugar and fodder beet were grown in three years to assess the potential sugar yield for ethanol production in the region. Fresh weight root yields ranged from 70-85 t/ha for sugar beet and 55-136 t/ha for fodder beet. Sugar contents averaged 15% for sugar beet and 10117o for fodder beet resulting in sugar yields of 10-12 t/ha and 5-12 t/ha respectively. Consistently higher sugar yield from suga...

White rot fungus Dichomitus squalens is an efficient lignocellulose degrading basidiomycete and a promising source for new plant cell wall polysaccharides depolymerizing enzymes. In this work, we focused on cellobiohydrolases (CBHs) of D. squalens. The native CBHI fraction of the fungus, consisting three isoenzymes, was purified and it maintained the activity for 60 min at 50°C, and was stable ...

The extraction of pectins from sugar beet pulp was carried out in an aqueous acid medium under different conditions using a full twostate experimental design for three extraction parameters (pH, temperature and time). The yields of the extracted pectins ranged from 4.1% to 16.2%. Their contents in pectin constituents were 35.2–76.3% galacturonic acid, 6.8–32.9% neutral sugars, 2.0–4.2% methoxy ...