Mutants of ECF1-ATPase were generated, containing cysteine residues in one or more of the following positions: aSer-411, bGlu-381, and eSer-108, after which disulfide bridges could be created by CuCl2 induced oxidation in high yield between a and e, b and e, a and g, b and g (endogenous Cys-87), and a and b. All of these cross-links lead to inhibition of ATP hydrolysis activity. In the two doub...
