Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...

I present an algorithm for computationally mixing screen colors (RGB colors) subtractively, written for a general audience. The question it addresses is, “Given two colors specified by their RGB triplets, what RGB triplet should be used to represent the color that would arise if the two colors were mixed like paint colors, i.e., mixed subtractively?” The only way I can think of doing this in a ...

The study is of brain activity in a blindsight subject (D.B.), who reports conscious visual afterimages of stimuli of which he is unaware when they are presented. This contrast offered a unique opportunity to study event-related potential recordings of conscious versus unconscious visual phenomena generated by the very same stimulus in the identical locus of the visual field. The behavioral res...

Traditionally, new loci involved in the Rhizobium-legume symbiosis have been identified by transposon mutagenesis and/or complementation. Wide dispersal of the symbiotic loci in Rhizobium species NGR234, as well as the large number of potential host-plants to be screened, greatly reduces the efficiency of these techniques. As an alternate strategy designed to identify new NGR234 genes involved ...

Cutting forces prediction is very important for cutting tool’s design and process planning. This paper presents a fuzzy cutting force modelling method using subtractive clustering for learning evaluation. In this method, subtractive clustering, combined with the least-square algorithm, identifies the fuzzy prediction model directly from the information obtained from the sensors. In the micro-mi...

A novel theory of subtractive hybridization including (or based on) the kinetic model of this process was proposed. A computer program modeling the process of subtraction was developed. Basing on the theory, a novel method of subtractive hybridization was proposed allowing routine comparison of genomes and products of genome expression. The method was applied to studies of the genetic mechanism...

Chiral Effective Field Theory ( $$\chi $$ EFT) has been extensively used to study the NN interaction during last three decades. In Theories (EFTs) renormalization is performed order by including necessary counter terms. Due strong character of a non-perturbative resummation needed. this work, we will review some methods proposed completely remove cut-off dependencies. The covered are with bound...

In this chapter, we take a different approach. In Section 2, we analyze the basics of analog subtractive synthesis, using the simple patch of a square-wave oscillator processed by a low-pass filter with a dynamically variable cutoff frequency. In Section 3, we show how a single mathematical function can model both the oscillator and filter together, and describe an efficient formation for this ...

Varieties like groups, rings, or Boolean algebras have the property that, in any of their members, the lattice of congruences is isomorphic to a lattice of more manageable objects: e.g. normal subgroups of groups, two-sided ideals of rings, …lters (or ideals) of Boolean algebras. Abstract algebraic logic can explain these phenomena at a rather satisfactory level of generality: in every member A...

Answering a question by Honsell and Plotkin, we show that there are two equations between λ-terms, the so-called subtractive equations, consistent with λ-calculus but not satisfied in any partially ordered model with bottom element. We also relate the subtractive equations to the open problem of the order-incompleteness of λ-calculus. 1998 ACM Subject Classification F.4.1 Lambda calculus and re...