We have developed a new strategy for immunization of wild rabbit populations against myxomatosis and rabbit hemorrhagic disease (RHD) that uses recombinant viruses based on a naturally attenuated field strain of myxoma virus (MV). The recombinant viruses expressed the RHDV major capsid protein (VP60) including a linear epitope tag from the transmissible gastroenteritis virus (TGEV) nucleoprotei...

CD8+ T cells have a critical role in antiviral immunity peripheral tissues, including skin. Using cutaneous vaccinia virus (VACV) infection model, we demonstrate that VACV immunization (with or with heterologous sequences) by skin scarification/ epidermal disruption (ss/ed) protected mice far more effectively against lethal respiratory challenge other viruses than routes of delivery intramuscul...

Abstract Background and Aims Urinary tract infections (UTI) are the most common of after renal transplantation with an estimated incidence between 10 98%. Recurrent urinary (rUTIs) increase mortality reduce graft survival transplantation. The present case describes immunization strategy against bacterial strains kidney Method We administered vaccine for prophylaxis rUTIs A transplant recipient ...

Abstract Coronaviruses have caused three deadly human outbreaks over the last 20 years, latest causing COVID-19 pandemic which has now lasted years. The animal reservoir of coronaviruses is extensive, and history shown that are able to crossover from animals humans. Thus, there a clear need for universal coronavirus vaccine. In this study, we produced S1 subunits spike proteins six different fu...

Vaccination is the best method to prevent spread of infectious diseases, its disadvantages are side effects. Potentially safe DNA, RNA or protein molecules possess antigenic properties, but low-immunogenic and therefore require conjugation with an adjuvant. The aim research was evaluate Chitosan (CS) potency as adjuvant compare effectiveness depending on route drug administration. experiments w...

The antibody response of Macaca fascicularis in parotid saliva and serum to local immunization by two routes with Streptococcus mutans was studied and compared over 1 year. Antibodies were titrated and classified by indirect immunofluorescent staining using specific antiglobulin conjugates. Antiglucosyltransferase activity was assayed by an enzyme inhibition test. Animals were immunized first b...

OBJECTIVES To assess the seroresponses to yellow fever vaccination at 6 and 9 months of age; assess any possible adverse effects of immunization with the 17D yellow fever vaccine in infants, particularly at 6 months of age. METHODS Four hundred and twenty infants who had completed BCG, OPV and DPT immunizations were randomized to receive yellow fever immunization at either 6 or 9 months. A si...

Despite the widespread use of Mycobacterium bovis BCG, the only licensed vaccine against tuberculosis (TB), TB remains a global epidemic. To assess whether more direct targeting of the lung mucosa by respiratory immunization would enhance the potency and longevity of BCG-induced anti-TB protective immunity, the long-term impact of intranasal (i.n.) BCG vaccination was compared to conventional s...

An attenuated nontoxinogenic nonencapsulated Bacillus anthracis spore vaccine expressing high levels of recombinant mutant protective antigen (PA), which upon subcutaneous immunization provided protection against a lethal B. anthracis challenge, was found to have the potential to serve also as an oral vaccine. Guinea pigs immunized per os with the recombinant spore vaccine were primed to B. ant...