The present study assessed the diagnostic usefulness of a multiplex PCR (mPCR) for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae applied to bronchoalveolar lavage (BAL). Fibreoptic bronchoscopy was performed on 156 hospitalised adult patients with lower respiratory tract infection (LRTI) and 36 controls. BAL fluid was analysed with bacteria...

into the selection frequency of resistant mutants and the bacterial kill rate by lev-ofloxacin and ciprofloxacin in non-mucoid Pseudomonas aeruginosa isolates from cystic fibrosis patients. In vitro pharmacodynamic modeling simulating free serum concentrations of fluoro-quinolones against multidrug-resistant Streptococcus pneumoniae. a novel fluoro-quinolone, activity against important respirat...

Aerobic In clusters ● Coagulase (+): Staphylococcus. aureus ● Coagulase (-):Staphylococcus lugdunensis or other coagulase-negative Staphylococcus In pairs/chains ● Optochin sensitive: Streptococcus pneumoniae ● Alpha-hemolytic: Viridans group Streptococcus, Enterococcus species ● Beta-hemolytic: ○ Group A Strep (Streptococcus pyogenes) ○ Group B Strep (Streptococcus agalactiae) ○ Group C, D, G ...

The prevalence and cotrimoxazole susceptibility of Streptococcus pneumoniae isolated from sputum of 100 HIV-positive patients attending the Nigeria Institute of Medical Research clinic was investigated using standard microbiological methods. Eleven of the sputum specimens grew Streptococcus pneumoniae. Antimicrobial susceptibility test showed that all the isolates were sensitive to amoxicillin,...

The pharmacokinetics of levofloxacin in serum and in skin blister fluid (SBF) was determined for 20 volunteers after a single 500-mg oral dose of levofloxacin. In addition, ex vivo bactericidal activity of SBF against Streptococcus pneumoniae and Staphylococcus aureus was studied. SBF containing levofloxacin and granulocytes killed 5.2 log of Streptococcus pneumoniae bacteria and 2.0 log of Sta...

We evaluated the applicability of ply PCR for confirmation of the identification of Streptococcus pneumoniae. lytA PCR, 16S rRNA sequencing, and amplified-fragment length polymorphism were used as reference methods. In contrast to the lytA gene, the ply gene proved to be not specific for S. pneumoniae. The presence of the ply gene in other streptococci, in particular Streptococcus mitis, sugges...

objective: in-time diagnosis of streptococcus pneumoniae (s. pneumonia) can play a significant role in decreasing morbidity and mortality rate. applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. examining the expression of different genes of this bacterium through different molecular methods suggests that lyta gene has a higher sensitivity...

The genes responsible for cell wall biosynthesis and cell division (dcw genes) were identified and sequenced in Streptococcus pneumoniae. The genetic organization of the dcw cluster in Streptococcus pneumoniae differed significantly from the clusters of other bacteria reported to date. In particular, the genes corresponding to the 2 min region of the Escherichia coli chromosome were found distr...

Sialylation of bacterial capsules has been proposed as an important virulence factor for several species of encapsulated pathogens, including group B Streptococcus. We have constructed a transposon mutant strain of type III group B Streptococcus that expresses a capsular polysaccharide differing from the wild type only in that the mutant strain's capsule lacks sialic acid. The mutant polysaccha...