Isolation of Apergillus flavus from groundnut and production of aflatoxin on rice. a method has been developed for the production of aflatoxin by growing Aspergillus flavus strain AJ on the solid substrate rice. Optimal yields, more than 1mg of aflatoxin B1 per gram of starting material, were obtained in 5 days at 28C.A crude product containing aflatoxin was isolated by HPLC (High Performance L...

three-hundred and twenty 7-day-old ross 308-strain broiler chickens were fed diets containing 0 or 125 ppb aflatoxin b 1 (afb1) from 7 to 28 days of age. sodium bentonite (0.5%), yeast (saccharomyces cervisiae) 0.2%, hydrated sodium calcium aluminosilicate (hscas) (0.5%), ammonia (0.5%), formycine (0.1%), and toxiban (0.1%) were added to the basal diet, as fed basis to determine the effects of ...

Aflatoxin contamination is a major economic and food safety concern for the peanut industry that largely could be mitigated by genetic resistance. To screen peanut for aflatoxin resistance, ten genotypes were infected with a green fluorescent protein (GFP)-expressing Aspergillus flavus strain. Percentages of fungal infected area and fungal GFP signal intensity were documented by visual ratings ...

aflatoxin m1 (afm1) is an important mycotoxin frequently found in milk and dairy products. dairy products and colostrum may be contaminated by aflatoxin m1 when dairy cattle have fed with aflatoxin b1-contaminated feeds. this study was undertaken in a dairy farm around tehran province to determine the presence and level of aflatoxin m1 (afm1) in spray dried and lyophilized colostrum samples. in...

In aflatoxin biosynthesis, the pathway for the conversion of 1-hydroxyversicolorone to versiconal hemiacetal acetate (VHA) to versiconal (VHOH) is part of a metabolic grid. In the grid, the steps from VHA to VHOH and from versiconol acetate (VOAc) to versiconol (VOH) may be catalyzed by the same esterase. Several esterase activities are associated with the conversion of VHA to VHOH, but only on...

AIMS The objective of this study was to determine if acetosyringone affected the expression of aflatoxin biosynthetic genes. METHODS AND RESULTS Two genes, nor1 and ver1, representing genes whose products are involved in early and late steps in aflatoxin biosynthesis, were examined. Two GUS (beta-glucuronidase) reporter constructs, nor1:GUS (pGAP12) and ver1:GUS (pGAP13), were used to study t...

DNA polymorphisms were used to infer relationships of a morphologically distinct new strain of aflatoxin-producing Aspergillus to other members of Aspergillus sect. Flavi. The new strain produced both Band G aflatoxins, and differed both morphologically and physiologically from other aflatoxin producers. Three isolates of the new fungus were compared with representatives of three known aflatoxi...

The present study was conducted to determine whether or not aflatoxin B1 was an effective inducing agent for lysogenic bacteria and to characterize some of the parameters involved in induction. A lysogenic strain of Bacillus megaterium (NRRL-B-3695) and an indicator strain of this species (NRRL-B-3694) were used. Cultures of the lysogenic strain were incubated for various periods of time in the...