The genome of Escherichia coli is composed of a single molecule of circular DNA with the length of about 47,000 kilobase pairs, which is associated with about 10 major DNA-binding proteins, altogether forming the nucleoid. We expressed and purified 12 species of the DNA-binding protein, i.e. CbpA (curved DNA-binding protein A), CbpB or Rob (curved DNA-binding protein B or right arm of the repli...

By introducing autonomous or software-controlled systems, human operators are increasingly required to perform cognitive-intensive tasks in addition existing labour-intensive tasks. As a result, it will be more difficult identify roles future complex systems with traditional approaches such as hierarchical task analysis used the conventional HRA. This paper proposes novel systematic approach fo...

Bacterial genomes sometimes contain genes that code for homologues of global regulators, the function of which is unclear. In members of the family Enterobacteriaceae, cells express the global regulator H-NS and its paralogue StpA. In Escherichia coli, out of providing a molecular backup for H-NS, the role of StpA is poorly characterized. The enteroaggregative E. coli strain 042 carries, in add...

The bacterial H-NS protein silences expression from sequences with higher AT-content than the host genome and is believed to buffer the fitness consequences associated with foreign gene acquisition. Loss of H-NS results in severe growth defects in Salmonella, but the underlying reasons were unclear. An experimental evolution approach was employed to determine which secondary mutations could com...

The genome of Escherichia coli is composed of a single molecule of circular DNA with the length of about 47,000 kilobase pairs, which is associated with about 10 major DNA-binding proteins, altogether forming the nucleoid. We expressed and purified 12 species of the DNA-binding protein, i.e. CbpA (curved DNA-binding protein A), CbpB or Rob (curved DNA-binding protein B or right arm of the repli...

The European Commission pursues a strategic action plan using the “Safe System” approach. function, layout and design of roads shall be coordinated in such way that human error is compensated, possible accidents no longer cause fatalities or serious injuries. Four fields are defined: people, vehicles, laws. This study aims to model process involved road safety management Austria based on System...

Los servicios de planificación financiera y la experiencia bancaria son desafíos sistemáticos diseño social tecnológico complicados, más que solo discutir números, dinero, inversiones beneficios. Por lo tanto, el propósito investigación es aplicar análisis procesos teóricos sistemas modificado (STPA) para estudiar banca minorista los financieros a través lente servicios, empoderarnos tener punt...

Abstract Whitby is the server-side of an Intelligent Tutoring System application for learning System-Theoretic Process Analysis (STPA), a methodology used to ensure safety anything that can be represented with systems model. The underlying logic driving reasoning behind Situation Calculus, which many-sorted situation, action, and object sorts. Calculus applied Ontology Authoring Contingent Scaf...

LeuO is a conserved and pleiotropic transcription regulator, antagonist of the nucleoid-associated silencer protein H-NS, and important for pathogenicity and multidrug resistance in Enterobacteriaceae. Regulation of transcription of the leuO gene is complex. It is silenced by H-NS and its paralog StpA, and it is autoregulated. In addition, in Escherichia coli leuO is antagonistically regulated ...