Pantothenate synthetase (EC 6.3.2.1), encoded by the panC gene, catalyzes the essential ATP-dependent condensation of D-pantoate and beta-alanine to form pantothenate in bacteria, yeast and plants. Pantothenate synthetase from Mycobacterium tuberculosis was expressed in E. coli, purified to homogeneity, and found to be a homodimer with a subunit molecular mass of 33 kDa. Initial velocity, produ...

Haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064 (DhaA) catalyzes the hydrolysis of carbon-halogen bonds in a wide range of haloalkanes. We examined the steady-state and pre-steady-state kinetics of halopropane conversion by DhaA to illuminate mechanistic details of the dehalogenation pathway. Steady-state kinetic analysis of DhaA with a range of halopropanes showed that bromopr...

This paper presents the analysis of a renewal input  finite buffer queue wherein the customers can decide either to  join the queue with a probability or balk. The service process is Markovian service process ($MSP$) governed  by an underlying $m$-state Markov chain. Employing the supplementary  variable and imbedded Markov chain techniques,   the steady-state system length distributions at pre...

One of the existing methods to build a confidence interval (c.i.) for the mean response in a single steady state simulation system is the batch means method. This method, compared to the other existing methods (autoregressive representation, regenerative cycles, spectrum analysis, standardized time series), is quite easy to understand and to implement and performs relatively well. However, the ...

This paper deals with a lumped-parameter dynamic simulation of a single-effect LiBr-H2O absorption chiller. In many studies the thermodynamic properties of LiBr-H2O solution were taken from some approximate relations causing the results to be somewhat inaccurate. These relations were used to solve simultaneous differential equations involving the continuity of species constituting the LiBr-H2O ...

Operability analysis as one of the most important bridges between process design and process control helps the process designer to investigate the control issues quite early in the design stage. Recently, Vinson and Georgakis suggested a steady state geometrical operability index as a quantitative measure for assessment of process operability. In...

flow in the first two-stage of v 94.2 gas turbine is simulated numerically. in this turbine, the second stator is clocked relative to the first stator to different positions. steady-state analysis was carried out by varying the circumferential relative position of the consecutive stator vanes to study the effects of the clocking on turbine performance. a density based compressible inviscid flow...

numerical solutions obtained by the meshless local petrov–galerkin (mlpg) method are presented for two-dimensional steady-state heat conduction problems. the mlpg method is a truly meshless approach, and neither the nodal connectivity nor the background mesh is required for solving the initial-boundary-value problem. the penalty method is adopted to efficiently enforce the essential boundary co...

nuclear cross sections that determine core multiplication strongly depend on core temperature (e.g., the doppler, moderator density effects etc). on the other hand, since this heat is generated by the neutron flux in the reactor core, the temperature distribution in the core will depend heavily on its neutronic behavior. fuel centerline temperature could be the limiting constraint on reactor po...