Ca2+ uptake and Ca2+-dependent ATP hydrolysis of fast skeletal muscle sarcoplasmic reticulum (SR) are strongly inhibited by trifluoperazine ('I"). Inhibition, which is Ca2+-dependent, is 90% with 14 p~ TFP and 0.2 p~ Ca2+. TFP interacts strongly, in a Ca2+-dependent way, with two SR proteins, calmodulin and the 53,000dalton glycoprotein. The two proteins were purified by TFP affinity chromatogr...

In addition to sculpting eukaryotic transcripts by removing introns, pre-mRNA splicing greatly impacts protein composition of the emerging mRNP. The exon junction complex (EJC), deposited upstream of exon-exon junctions after splicing, is a major constituent of spliced mRNPs. Here, we report comprehensive analysis of the endogenous human EJC protein and RNA interactomes. We confirm that the maj...

Serine/arginine-rich proteins (SR proteins) constitute a family of RNA-binding proteins conserved throughout metazoans. The SR proteins are essential for constitutive pre-mRNA splicing and also affect regulated pre-mRNA splicing. We identified five putative genes encoding SR proteins (referred to as srp genes) in Caenorhabditis elegans, examined their expression using the gfp gene as a reporter...

Global analyses of splicing of precursor messenger RNAs (pre-mRNAs) have revealed that alternative splicing (AS) is highly pervasive in plants. Despite the widespread occurrence of AS in plants, the mechanisms that control splicing and the roles of splice variants generated from a gene are poorly understood. Studies on plant serine/arginine-rich (SR) proteins, a family of highly conserved prote...

Small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP splicing factors containing a serine/arginine-rich domain (SR proteins) concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. Nuclear SFCs are considered mainly as storage sites for splicing factors, supplying splicing factors to active genes. The mechanisms controlling the interaction of the v...

plicing factors, usually associated with RNA polymerase II transcripts, have a temporary home near sites of RNA polymerase I transcription, as shown on page 51 by Bubulya et al. During interphase, premRNA splicing factors such as the serine arginine–rich (SR) proteins reside in nuclear speckles, along with other pre-mRNA processing proteins. The speckles disassemble during mitosis, and then ref...

plicing factors, usually associated with RNA polymerase II transcripts, have a temporary home near sites of RNA polymerase I transcription, as shown on page 51 by Bubulya et al. During interphase, premRNA splicing factors such as the serine arginine–rich (SR) proteins reside in nuclear speckles, along with other pre-mRNA processing proteins. The speckles disassemble during mitosis, and then ref...

plicing factors, usually associated with RNA polymerase II transcripts, have a temporary home near sites of RNA polymerase I transcription, as shown on page 51 by Bubulya et al. During interphase, premRNA splicing factors such as the serine arginine–rich (SR) proteins reside in nuclear speckles, along with other pre-mRNA processing proteins. The speckles disassemble during mitosis, and then ref...