Peripheral blood stem cells and progenitor cells, collected during recovery from exposure to cytotoxic agents or after cytokine administration, are being increasingly used in clinical bone marrow transplantation. To determine factors important for mobilization of both primitive stem cells and progenitor cells to the blood, we studied the blood and splenic and marrow compartments of intact and s...

We investigated the effects of B cell stimulatory factor 2/interleukin 6 (BSF-2/IL-6) on the development of murine hemopoietic progenitors using serum-containing culture and serum-free culture. In serum-containing culture, BSF-2 mainly supported multipotential blast cell colonies from spleen cells of normal and 5-fluorouracil (5-FU)-treated mice. In serum-free culture, no colony growth was seen...

Two distinct clones of Friend spleen focus-forming virus (SFFV), differing in their erythroleukemic potential, are described. These isolates have been cloned free of their associated helper viruses and shown to be replication-defective. Both SFFV isolates have been rescued from rat fibroblast nonproducer cell clones with cloned replication-competent viruses, F-MuLVA and F-MuLVP, obtained from t...

The treatment of mice with high doses of 5-fluorouracil (5-FU) results in an enrichment of primitive hematopoietic progenitors. Using this procedure, we obtained a new class of murine hematopoietic colonies that had very high secondary plating efficiencies in vitro and could differentiate into not only myeloid cells but also into lymphoid lineage cells. The phenotypes of interleukin-3 (IL-3) in...

The genetically anemic W/W° mice are characterized by the inability of their bone marrow cells to form macroscopic pluripotent hematopoietic colonies in the spleen ofirradiated recipients upon transfer (colony-forming units) . Furthermore, they almost totally lack mast cells, notably in the skin . In the present study, we have tested the effect of recombinant murine interleukin 3 (rmIIr3) on W/...

-Characteristics of the AKB6F1 mouse strain make it suitable as an assay animal for quantitating haemopoietic stem cells, or spleen colony-forming units (CFU-S), from leukaemic AKR mice. Marrow cells harvested from leukaemic mice were assayed for CFU-S in lethally irradiated AKR or AKB6F1 hosts. Survival times and numbers of leukaemic colony-forming units (L-CFU) in unirradiated recipients were...

A large variability in the stem cell by colony development in sublethally content of the spleens of female mice irradiated female mice. It is shown by comparison with that of the bone that, for mice in pre-estrus, the spleens marrow, or with that of the spleen contain up to three times as many and bone marrow of male mice, is hemopoietic stem cells (CFU) as durdemonstrated. This variability is ...

A large variability in the stem cell by colony development in sublethally content of the spleens of female mice irradiated female mice. It is shown by comparison with that of the bone that, for mice in pre-estrus, the spleens marrow, or with that of the spleen contain up to three times as many and bone marrow of male mice, is hemopoietic stem cells (CFU) as durdemonstrated. This variability is ...

Slj/+ mice display a slight macrocytic anaemia due to a defect in their haemopoietic organ stroma. They have a deficient endogenous spleen colony (CFU-end) formation following sublethal doses of gamma-radiation compared with their normal +/+ littermates, which is likely to be due to the low pre-irradiation CFU-S content of the Slj/+ spleen. CFU-S in these congenic mice do not differ in their se...

The genetically anemic W/Wv mice are characterized by the inability of their bone marrow cells to form macroscopic pluripotent hematopoietic colonies in the spleen of irradiated recipients upon transfer (colony-forming units). Furthermore, they almost totally lack mast cells, notably in the skin. In the present study, we have tested the effect of recombinant murine interleukin 3 (rmIL-3) on W/W...