in order to investigate the effect of two spermidine concentrations (0.5 and 1 µ m ) on in vitro gynogenesis of six onion accessions from khorasan province this research was carried out. results showed that the highest and lowest percentage of gynogenesis rate was belonged to m2 and m5 media, respectively. the highest (1.41) and lowest (0.33) percentage of gynogenic embryo were belonged to rosh...

to study the effects of postharvest treatments of spermidine and putrescine on storage quality of ‘hayward’ kiwifruit, the present research was performed. in november 2010, fruits were hand picked from fajr orchardist corporation in sari and transported to postharvest physiology laboratory. fruits were dipped for 4 minutes in either spermidine or putrescine solutions (0.5, 1.0 and 1.5 mm), and ...

Most of the spermidine in Escherichia coli is converted to glutathionylspermidine at the end of logarithmic growth. Methods are presented for the determination and isolation of glutathionylspermidine and for its characterization as y-glutamylcysteinylglycylspermidine. Isotopic experiments demonstrate that the spermidine of glutathionylspermidine is in equilibrium with free intracellular spermid...

in this research, we studied polyamines including putrescine (put), spermidine (spd) and spermine (spm) changes in three common bean (phaseolus vulgaris) genotypes, k-s-31167, ge-288 and naz under drought stress. our result showed steadily increase in putrescine (put) and spermine (spm) in all three genotypes.  ge-288 genotype showed decrease and then increasing in spermidine (spd) content but ...

Escherichia coli CAG2242 cells are deficient in the speG gene encoding spermidine acetyltransferase. When these cells were cultured in the presence of 0.5 to 4 mM spermidine, their viability was greatly decreased through the inhibition of protein synthesis by overaccumulation of spermidine. When the cells were cultured with a high concentration of spermidine (4 mM), a revertant strain was obtai...

BACKGROUND Recently, tea (Camellia sinensis) flowers have attracted increasing interest because of their content of bioactive compounds such as catechins. The aim of this study was to investigate the occurrence of some characteristic compounds in tea flowers. RESULTS A principal component analysis of metabolites using ultra-performance liquid chromatography/time-of-flight mass spectrometry sh...

Deoxyhypusine synthase catalyses the first step in the biosynthesis of hypusine [N(epsilon)-(4-amino-2-hydroxybutyl)lysine]. The crystal structure of human deoxyhypusine synthase in complex with NAD revealed four NAD-binding sites per enzyme tetramer, and led to a prediction of the spermidine-binding pocket. We have replaced each of the seven amino acid residues at the predicted spermidine-bind...

BackgroundThe cross-talk between the host and its microbiota plays a key role in promotion of health. The production metabolites such as polyamines by intestinal-resident bacteria is part this symbiosis shaping immunity. putrescine, spermine, spermidine are abundant within gastrointestinal tract might substantially contribute to gut immunity.ObjectiveWe aimed characterize polyamine modulator T-...

Polyamines are ubiquitous biologically active aliphatic cations that are at least transiently available in the soil from decaying organic matter. Our objectives in this study were to characterize polyamine uptake kinetics in Phytophthora sojae zoospores and to quantify endogenous polyamines in hyphae, zoospores, and soybean roots. Zoospores contained 10 times more free putrescine than spermidin...

BACKGROUND Spermidine is a dietary polyamine that is able to activate protein tyrosine phosphatase non-receptor type 2 (PTPN2). As PTPN2 is known to be a negative regulator of interferon-gamma (IFN-γ)-induced responses, and IFN-γ stimulation of immune cells is a critical process in the immunopathology of inflammatory bowel disease (IBD), we wished to explore the potential of spermidine for redu...