OBJECTIVE To evaluate and compare standard sperm parameters, lipid peroxidation of sperm plasma membranes, antioxidant capacity of seminal plasma, and sperm chromatin integrity in ejaculates from men whose partners have a history of recurrent pregnancy loss and from a control group of men with recent fertility. DESIGN Descriptive study. SETTING Reproduction Program, University of Antioquia,...

The swim-up procedure was used to collect a sperm fraction with improved motility and normal morphology from fertile and infertile semen samples. The untreated and swim-up fractions were analyzed with the Hamilton-Thorn Motility Analyser. Comparisons were made to see the effect of this sperm selection technique on sperm motility and morphology. They were then examined by transmission elect...

The efficiency of sperm capacitation and of the acrosome reaction was studied in the teratospermic domestic cat to evaluate further the etiology of compromised zona pellucida penetration and oocyte fertilization. Specific objectives were to compare normospermic and teratospermic cat ejaculates for 1) the kinetics and timing of sperm capacitation in vitro as determined by an ionophore-induced ac...

The purpose of this study was to examine if selecting a sperm population with improved motion characteristics before freezing reduces the deleterious effects of cryopreservation. Semen specimens from 15 normal donors were divided into two equal aliquots. The first aliquot received no treatment (control), and the second was processed by swim-up from a washed sperm preparation to select a sperm p...

BACKGROUND Intrauterine insemination (IUI) is a valid treatment for infertility with a cumulative pregnancy rate of >40-90% after 3-10 treatment cycles. We studied the efficacy of a simplified method for motile sperm preparation for IUI. METHODS A prospective clinical trial was performed with 100 couples (male age 33-48 and female 28-37 years) with a 2-8 year history of primary infertility as...

the swim-up procedure was used to collect a sperm fraction with improved motility and normal morphology from fertile and infertile semen samples. the untreated and swim-up fractions were analyzed with the hamilton-thorn motility analyser. comparisons were made to see the effect of this sperm selection technique on sperm motility and morphology. they were then examined by transmission electron m...

background: leptin is a polypeptide hormone secreted by white adipose tissue in proportion to body energy. although the participation of leptin in female reproduction is well established any role in male reproductive function is at best tenuous. objective: the objective of this study was to compare the leptin concentration in human seminal plasma and then the relationships between seminal lepti...

BACKGROUND There are no studies relating the apoptotic marker caspase-3 in human sperm to different degrees of abnormal sperm concentration, morphology and rapid progressive motility. METHODS Semen from 67 males with abnormal semen analyses (n = 61) and normozoospermia (n = 6) were used. In each case, sperm from the neat semen (semen fraction) and after gradient centrifugation and swim-up (sw...

Background: Lipopolysaccharide (LPS) is a major component of Chlamydia spp, and of all Gram-negative bacteria. The role of LPS in alteration of sperm parameters is controversial. In this study quantification of Chlamydia-specific and general lipopolysaccharide in semen was correlated to semen quality. Materials and Methods: 255 male partners of infertile couples were recruited to produce urine ...

Abstract Study question Is our novel deviceless method based on in-situ microfluidics a valuable strategy to select suitable sperm for ICSI? Summary answer The protocol allows selecting ICSI within 15 minutes, in the same dish and disregarding fungible supplies, centrifugation washing steps. What is known already Microfluidics an innovative operation ART which integrates guidance biomimicry dur...