Infertility is a common yet complex problem affecting approximately 10-15% of couples attempting to conceive a baby. Especially, 40-50% of these factors are known as male-related disorders. Unlike female infertility, the cause of which is often easily identified, diagnosing male factors can be difficult. Male infertility is due to low sperm production, abnormal sperm function or blockages of sp...

Despite the advent of ICSI, cases of total fertilization failure (TFF) often lead to cycle cancellation with limited diagnostic and therapeutic strategies currently available. We report on the case of an infertile couple who failed to conceive after repeated IVF and ICSI. Sperm of the husband were morphologically normal and passed a functional test assessing their ability to activate mouse oocy...

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic H...

the liver is an organ at risk (oar) in radiotherapy of thoracic and abdominal tumors such as gastric, distal esophagus, lower lung and breast, bile duct, pancreas and whole abdomen. in this study the alteration in liver functional tests (lft) of these patients during radiotherapy was investigated. to that end, the level of serum aspartate aminotransferase (ast), alanine aminotransferase (alt), ...

This study was conducted to examine if L-carnitine supplementation in the Tris-egg-yolk extender can improve the motility characteristics, membrane integrity, and in vitro fertilization potential after cryopreservation of ejaculated bovine spermatozoa. It was carried out on January to May 2013 at the University of Wisconsin-Madison. L-carnitine at final concentrations of 0.5, 1, 10, and 30mM we...

ABSTRACT: Spermatozoa experience oxidative, osmotic, chemical, and thermal stresses when cooled, which degrade the quality fertilizing capacity of cells. Adding antioxidants to sperm extender mitigates these alterations. This study evaluated effect isoespintanol (ISO) on boar semen subjected cooling. Fifteen ejaculates from five boars (Susscrofadomestica) were extended in Beltsville thawing sol...

Recently, the conception rates after artificial insemination have been pointed out to decline continuously. To overcome this problem, the control of frozen and thawed sperm quality is required. However, the mechanism of bovine sperm functional regulation is still largely unknown. In mammals, the ejaculated sperm are capable of showing fertilizing ability during migration in the female reproduct...

BACKGROUND Sperm DNA integrity (SDI) is an important factor in the prognosis of male fertility. Here we compare the toluidine blue (TB) image cytometry test, recently proposed by us for SDI assessment, with two other tests-the sperm chromatin structure assay (SCSA) and the terminal nick-end labelling (TUNEL) assay. METHODS Sperm samples from 35 men were evaluated for standard sperm parameters...

Sperm genomic integrity and ultrastructural features of ejaculated spermatozoa contributing to the assessment of gamete fertility potential in patients with asthenozoospermia are discussed. The proportion of TUNEL-positive cells was significantly higher in the semen of patients with low sperm motility (n=40; p<0.01) as compared to men with normal sperm motility (n=54). Sperm DNA fragmentation n...

A wide variety of techniques for the preparation of sperm are currently available, of which the most commonly employed are density-gradient centrifugation (DGC) and swim-up (SUP). To date, these methods appear to be effective in selecting functional sperm for assisted reproduction techniques (ART), but they may have negative effects on sperm DNA. In this study, the ability of these semen proces...