background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...

Background The aim of present study is to evaluate the effect of PolyVinyl Pyrrolidone (PVP) routinly used during ICSI procedure on sperm membrane integrity, and sperm chromatin status. MaterialsAndMethods This study was carried out on 21 semen samples from the infertile men referring to Isfahan Fertility and Infertility Center. The processed semen samples were divided into two portions. One po...

Background: During spermatogenesis the sperm nucleus exchange histones against cystein rich protamines, which stabilize the sperm chromatin and protects the DNA against damages. At ejaculation the age of the patient, handling routines, processing of the semen sample and the storage of the final sperm suspension might damage the DNA. Sperm chromatin stability and DNA damages are evaluated with d...

The relationship between sperm nuclear shape and bull fertility was determined. Two groups of bulls, 3 per group, were selected. Bulls differed in fertility based on lifetime nonreturn rates. Digital images of propidium iodide-stained sperm from each bull were collected and shape-evaluated by Fourier harmonic amplitudes 0 to 5. A discriminant function (P < .05) was constructed based on harmonic...

OBJECTIVE To determine the consequences of adjuvant testicular germ cell tumor treatment (TGCT) on sperm characteristics and sperm DNA, and to evaluate the predictors of sperm recovery. DESIGN Multicenter prospective longitudinal study of patients analyzed before treatment and after 3, 6, 12, and 24 months. SETTING University hospitals. PATIENT(S) One hundred twenty-nine volunteer TGCT pa...

BACKGROUND Diabetes mellitus has a variety of structural and functional effects on the male reproductive system. Diabetes results in reduced sperm parameters and libido. The present study aims to investigate the effects of royal jelly (RJ) on reproductive parameters of testosterone and malondialdehyde (MDA) production in diabetic rats. MATERIALS AND METHODS This experimental study was conduct...

Background: Diabetes mellitus (DM), primary or idiopathic, is a chronic disorder of the carbohydrate, lipid and protein metabolism, characterized through hyperglycemia and glycosuria. DM can involve male reproductive function at many levels. Vitamin C ( ascorbic acid) is one of necessary elements in human and mammalian diet that is very important in fertility. Vitamin C prevent from gamets dama...

BACKGROUND The compaction of human sperm chromatin is the result of replacement of approximately 85% of histones with protamines. Germ-line testis/sperm-specific histone 2B (TSH2B) has been detected in only approximately 30% of mature spermatozoa. Its level in the semen of subfertile patients varies; its function is unknown. We evaluated TSH2B in the sperm samples of 23 donors and 49 subfertile...

The technique (sperm chromatin structure assay) used in this study identifies one reason (a decrease in chromatin quality) for stallions whose semen shows reduced fertility when chilled. This assay is a diagnostic test, in addition to the use of sperm motility and morphologic evaluation, to evaluate the quality of chilled stallion semen and further aid the practitioner and the breeding industry...

Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...