effects of different levels of cadmium (0.1, 1, 2, 4, 8, 16, 32, 64, 128, 256 mg/l of culture medium) were evaluated using gas production technique. in the glass vials used to measure gas production 30 mg of buffered rumen fluid (2:1 ratio of rumen fluid: buffer) poured in glass and cultured at 38.6 temperature. volume and pressure of produced gas were measured with syringes and digital pressur...

Introduction T h e first axiom of ruminology appears to be: ‘The rumen is a strictly anaerobic system’ (Hungate, 1966) but before we accept this we should examine the evidence for and against this assertion. Probably the strongest evidence for anaerobiosis comes from the studies of the reactions in the rumen, from the stoichiometries observed and from the low oxidation-reduction potential obtai...

Lignocellulose is an abundant and renewable resource that holds great promise for sustainable bioprocessing. However, unpretreated lignocellulose is recalcitrant to direct utilization by most microbes. Current methods to overcome this barrier include expensive pretreatment steps to liberate cellulose and hemicellulose from lignin. Anaerobic gut fungi possess complex cellulolytic machinery speci...

Ruminants fulfill their energy needs for growth primarily through microbial breakdown of plant biomass in the rumen. Several biotic and abiotic factors influence the efficiency of fiber degradation, which can ultimately impact animal productivity and health. To provide more insight into mechanisms involved in the modulation of fibrolytic activity, a functional DNA microarray targeting genes enc...

An examination of the fecal microflora of adult swine was made with regard to the efficiency of several roll tube media in enumeration and recovery of anaerobes, the effects of medium constituents on recovery, and the isolation and identification of the predominant kinds of bacteria. Total number of organisms by microscopic bacterial counts varied among fecal samples from 4.48 X 10(10) to 7.40 ...

Abstract The rumen harbours countless bacteria, archaea, ciliated protozoa, fungi and viruses: various microorganisms that have established multiplicity of relationships to efficiently digest complex plant fibres polysaccharides produce volatile fatty acids (VFAs), microbial proteins vitamins, essentials for the host’s health, growth performances. Recent studies using omics-based techniques rev...

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