The antigenicity of liposomes sensitized with haptenated phosphatidylethanolamine (PE) and the reactivity of the liposomes with complement depended on the length of the spacer between hapten and PE. To establish the optimal conditions for the assay, haptenated PE's with various length of spacers are required. In the previous method, hapten-spacer molecule was first synthesized to which PE was c...

The spacer A/T region is a positive cis-acting DNA element that was identified in the Chlamydia trachomatis rRNA promoter region. We have now demonstrated that similar sequences in other chlamydial promoters are important for transcription. Substitution of candidate spacer A/T regions in four chlamydial promoters decreased transcription by partially purified C. trachomatis RNA polymerase in an ...

Background: Periprosthetic Joint Infection (PJI) is a dreadful complication of primary Total Hip Arthroplasty (THA). Following revision THA, up to 17% THA can be complicated with PJI. Three-stage should only done if simple debridement fails treat Here we present rare case persistent PJI, treated three-stage and bone grafting.Case Report: A thirty-seven-year-old female patient came chief complai...

D iaphyseal defects too long to be bridged by cancellous bone graft require complex reconstruction. Distraction osteogenesis requires specialized equipment, has a steep learning curve, and is plagued by attendant pin-site complications and nonunion. Vascularized bone, such as from the fibula, requires microsurgical anastomoses (free), or is limited by pedicle length (pedicled), and has attendan...

A series of sorbents containing various amounts of borate groups were prepared by chemical immobilization ofP-aminoethylphenylboric acid on a copolymer- treated organosilane spacer glass plates. It was found thatbaker's yeast cells selectively adhered to these supports. Yeast cells stained with procion red HE-3B formeddenser and more stable layer on the copolymer-grafted supports compared onto ...

Within the scope of Collaborative Research Centre SFB 639 "Textile-reinforced composite components for function-integrating multi-material design in complex lightweight applications", woven and flat-knitted spacer fabrics from commingled hybrid yarns are developed, pressed and the characteristics of the formed composite structures are tested. The developed spacer fabric structures have Ushaped ...

Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...

RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...

Nuclear factor I (NFI) binds tightly to DNA containing the consensus sequence TGG(N)6-7GCCAA. To study the role of the spacing between the TGG and GCCAA motifs, oligonucleotides homologous to the NFI binding site FIB-2 were synthesized and used for binding assays in vitro. The wild-type site (FIB-2.6) has a 6bp spacer region and binds tightly to NFI. When the size of this spacer was altered by ...

The intergenic spacer region between the 16S and 23S rRNA genes of Mycoplasma haemomuris, previously classified as 'Haemobartonella muris', was amplified by PCR and sequenced for analysis of the primary and secondary structures of the RNA transcript. The spacer region consisted of 219 base-pairs and lacked the spacer tRNA gene. A hypothetical secondary structure predicted in the RNA transcript ...