Intracellular levels of coenzyme A (CoA) and its derivatives involved in the metabolic pathways for Clostridium acetobutylicum ATCC 824 were analyzed by using reverse-phase high-performance liquid chromatography (HPLC). During the shift from the acidogenic to the solventogenic or stationary growth phase, the concentration of butyryl-CoA increased rapidly and the concentrations of free CoA and a...

The structures of three mutants of bacteriophage T4 lysozyme selected using a screen designed to identify thermostable variants are described. Each of the mutants has a substitution involving threonine. Two of the variants, Thr 26-->Ser (T26S) and Thr 151-->Ser (T151S), have increased reversible melting temperatures with respect to the wild-type protein. The third, Ala 93-->Thr (A93T), has esse...

The synthesis of two series N-annulated perylene bisimides (PBIs), compounds 1 and 2, is reported, their self-assembling features are thoroughly investigated by a complete set spectroscopic measurements theoretical calculations. study corroborates the enormous influence that distance between PBI core peripheral groups exerts on chiroptical properties supramolecular polymerization mechanism. Com...

Solvent-tolerant microbes have the unique ability to thrive in presence of organic solvents. The present study describes the effect of increasing hydrophobicity (log Pow values) of organic solvents on the outer membrane proteome of the solvent-tolerant Pseudomonas aeruginosa PseA cells. The cells were grown in a medium containing 33% (v/v) alkanes of increasing log Pow values. The outer membran...

The electronic absorption profiles of 3-cyano-1,5-diarylformazans were recorded in different organic solvents having different polarities. Both macroscopic and microscopic aspects were contemplated to determine the influence of various solute-solvent interactions upon absorption spectral shifts. The effects of solvent polarity on the absorbance profiles of the compounds were evaluated. The mult...

Extending the knowledge on secondary metabolites and the effect of solvent of extraction in mushrooms, We investigated five mushrooms species; two cultivated edible (Agaricus bisporus (brown) and Agaricus bisporus (white); and three wild medicinal edible mushrooms Trametes versicolor, Ganoderma applanatum and Fomes fomentarius for total phenolic, total flavonoid and condensed tannins content. T...

We review recent developments and applications of aldolase-type organocatalytic direct transformations in aqueous media without addition of organic solvent.

This paper aims to provide a review of the analytical extraction techniques for polycyclic aromatic hydrocarbons (PAHs) in soils. The extraction technologies described here include Soxhlet extraction, ultrasonic and mechanical agitation, accelerated solvent extraction, supercritical and subcritical fluid extraction, microwave-assisted extraction, solid phase extraction and microextraction, ther...