A high molecular weight actin-binding protein was isolated from the Physarum polycephalum plasmodia. The protein ( HMWP ) shares many properties with other high molecular weight actin-binding proteins such as spectrin, actin-binding protein from macrophages, and filamin. It has a potent activity to cross-link F-actin into a gel-like structure. Its cross-linking activity does not depend on calci...

Atomistic simulations are used to characterize the molecular dynamics (MD) of alkyl chains with different functionalizations in different water/acetonitrile mixtures (80/20 and 50/50). Starting from fully equilibrated solvent systems (flat density profile for both components), microheterogeneous structuring of the solvent in the chromatographic system is found for both mixtures. Depending on th...

The quantum yield, under non-photoreactivating conditions, for inactivation of tobacco mosaic virus RNA by ultraviolet radiation (254 nm.) is independent of the RNA concentration but varies appreciably with the ionic strength of the irradiation solvent, being approximately three times greater in water than in o-I M-potassium phosphate buffer. Photoreactivation of inactivated tobacco mosaic viru...

The optical rotatory dispersion of L-tryptophan, L-tyrosine, L-phenylalanine, and some of their derivatives was investigated in the ultraviolet region as a function of solvent, pH, and temperature. All aromatic transitions accessible to study were found to be optically active, but the rotatory strength was found to be low. The long wave length tyrosine bands at 280 rnp showed a loo-fold lower r...

The optical rotatory dispersion of L-tryptophan, L-tyrosine, L-phenylalanine, and some of their derivatives was investigated in the ultraviolet region as a function of solvent, pH, and temperature. All aromatic transitions accessible to study were found to be optically active, but the rotatory strength was found to be low. The long wave length tyrosine bands at 280 rnp showed a loo-fold lower r...

Functional tissue engineering of load-bearing repair tissues requires the design and production of biomaterials that provide a remodelable scaffold for host infiltration and tissue regeneration while maintaining the repair function throughout the remodeling process. Layered constructs have been fabricated from chemically and mechanically cleaned porcine intestinal collagen using ethyl-3(3-dimet...

Aqueous processing of globular protein-polymer diblock copolymers into solid-state materials and subsequent solvent annealing enables kinetic and thermodynamic control of nanostructure formation to produce block copolymer morphologies that maintain a high degree of protein fold and function. When model diblock copolymers composed of mCherry-b-poly(N-isopropylacrylamide) are used, orthogonal con...

Ionic liquids are novel solvents of interest as greener alternatives to conventional organic solvents aimed at facilitating sustainable chemistry. As a consequence of their unusual physical properties, reusability, and eco-friendly nature, ionic liquids have attracted the attention of organic chemists. Numerous reports have revealed that many catalysts and reagents were supported in the ionic l...

The acid–base properties of calcon (1-(2-hydroxy-1-naphthylazo)-2-naphthol-4-sulfonic acid) in water and mixed water–organic solvents at 25 °C at an ionic strength of 0.10 M are studied by a multiwavelength spectrophotometric method. The organic solvents used were the amphiprotic (methanol), dipolar aprotic (dimethylsulfoxide), and low basic aprotic (acetonitrile). To evaluate the pH absorbance...

_I BHARMACIA GRADIENT MIXER GM-1 The prime requirement for the production of linear ionic strength gradients is efficient mixing of the components of the gradient. In the Pharmacia Gradient Mixer GM-1 this is achieved by a blade configuration which lifts the dense incoming solution from the bottom of the mixing chamber and distributes i t evenly throughout the whole eluant at a low stirring spe...