A l*C-diterpene alcohol (copalol) was isolated as its 4-(4’nitrophenylazo)benzoyl ester from a soluble enzyme system which had been prepared from a cell-free extract of mycelia of the fungus Gibberella fujikuroi and incubated with ZJ*Cmevalonate, ATP, and MgC12. Copalol was identified as trans-( -)-labda-8(16), 13-dien-15-01 from its properties, in comparison with those of the enantiomeric alco...

The availability of water and nutrients are determining factors for the growth productivity cucumber crop. implementation grafting techniques increases efficiency in absorption resources such as nutrients, improving quality, mineral content, enzymatic activity fruit. objective this research work was to evaluate effect anchor point (soil substrate), graft (with without graft), irrigation volume ...

Many cellular reactions involve both hydrophobic and hydrophilic molecules that reside within the chemically distinct environments defined by the phospholipid-based membranes and the aqueous lumens of cytoplasm and organelles. Enzymes performing this type of reaction are required to access a lipophilic substrate located in the membranes and to catalyze its reaction with a polar, water-soluble c...

1. Liver cell fractions were prepared by non-aqueous procedures and nuclei were also obtained in a hyperosmotic sucrose medium. Histone phosphokinase activity, assayed with histone F1 as substrate, was present in the soluble fraction of the cytoplasm and also bound on to the chromatin fraction of the nucleus. 2. The activity of the enzyme increased sixfold in nuclei from regenerating livers 22h...

The specific activities of highly purified preparations of human parotid and pancreatic amylase on several different soluble and insoluble starches were compared. The ratio of parotid to pancreatic activity varied with the physical nature of the substrate. With all soluble starches, irrespective of source, parotid amylase exhibited a higher specific activity than did pancreatic amylase; the rev...

Membrane-integrated type II phosphatidic acid phosphatases (PAP2s) are important for numerous bacterial to human biological processes, including glucose transport, lipid metabolism, and signaling. Escherichia coli phosphatidylglycerol-phosphate phosphatase B (ecPgpB) catalyzes removing the terminal phosphate group from a lipid carrier, undecaprenyl pyrophosphate, and is essential for transport ...

In a soilless culture (perlite substrate), root restriction cannot only reduce production costs but also improve fruit quality. Therefore, this study used different levels of [T1: 0.5 L, T2: 4 nonrestriction treatment (CK): 35 L] on tomatoes to explore their impact Results showed that total soluble solids (TSS), glucose, fructose, and sucrose contents were increased, whereas L-tryptophan, L-tyr...

Agricultural food waste is rich in cellulosic and non-cellulosic fermentable substance. In this study, we investigated the bioconversion of pineapple cell wall sugars into bioethanol by simultaneous saccharification fermentation using Saccharomyces cerevisiae ATCC 4126. Soluble insoluble were during process. Moreover, medium was for protein, moisture, ash, lignin glycerol determinations with a ...

UDP-Glucuronosyltransferases (UGTs) are a class of enzymes (please refer to Mackenzie et al. [1] for UGT nomenclature and classification) anchored in the membrane of endoplasmic reticulum. The UGT enzyme catalyzes glucuronidation, a major metabolic pathway in humans, by transferring a glucuronic acid from the cofactor UDPglucuronic acid to the substrate. In vitro characterization has revealed t...