background: the mainstay of diagnosis of relapsing fever (rf) is demonstration of the spirochetes in giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. this study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. methods: animal blood sampl...

background and objectives : in iran, anaplasmosis is normally diagnosed with traditional giemsa staining method. this is not applicable for identification of the carrier animals. the aim of this study was to compare the detection of anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional giemsa staining method compared with the pcr-rflp technique. mate...

a total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in mashhad. the presence of salmonella was assessed by conventional culture method and confirmed by using poly o and poly h antiserum in serotyping. pcr amplification of inva gene as a specific method for detection of salmonella was...