The polyphosphate kinase gene from Pseudomonas aeruginosa was overexpressed in its native host, resulting in the accumulation of 100 times the polyphosphate seen with control strains. Degradation of this polyphosphate was induced by carbon starvation conditions, resulting in phosphate release into the medium. The mechanism of polyphosphate degradation is not clearly understood, but it appears t...

Vibrio cholerae, the causative agent of Asiatic cholera, has been reported to make large quantities of polyphosphate. Inorganic polyphosphate is a ubiquitous molecule with a variety of functions in prokaryotic and eukaryotic cells. We constructed a V. cholerae mutant with a deletion in the polyphosphate kinase (ppk) gene. The mutant was defective in polyphosphate biosynthesis. Deletion of ppk h...

In the U.S. market place, there are many examples of precooked poultry products designed to be reheated in a microwave oven and, to a lesser extent, fish products such as tilapia. However, few U.S. catfish products are designed to be microwave cooked or reheated. The first objective of this study was to examine the properties of microwave cooked raw frozen catfish fillets and oven precooked (to...

Phosphorus removal in waste stabilization ponds (WSP) is highly variable, but the reasons for this are not well understood. Luxury uptake of phosphorus by microalgae has been studied in natural systems such as lakes but not under the conditions found in WSP. This work reports on the effects of phosphate concentration, light intensity, and temperature on luxury uptake of phosphorus by WSP microa...

Quantitative electron microscopy (stereology) was used to assess the ultrastructural response of three algae representative of the classes Chlorophyceae, Cyanophyceae, and Bacillariophyceae to lead-coupled polyphosphate degradation. The organisms were exposed to a culture medium concentration of 20 ppb Pb for 3 hr at the time of luxury phosphate uptake and subsequently transferred to phosphorus...

To evaluate the utility of polyphosphate kinase gene (ppk)-specified polyphosphate in mercury remediation, a fusion plasmid, pMK27, with ppk from Klebsiella aerogenes and mercury transport genes, merT and merP, from Pseudomonas K-62, was constructed. The transcription and translation of ppk, merT and merP were found to be mercury-inducible. The ppk-specified polyphosphate was identified in cell...

Arbuscular mycorrhizal (AM) fungi accumulate a massive amount of phosphate as polyphosphate to deliver to the host, but the underlying physiological and molecular mechanisms have yet to be elucidated. In the present study, the dynamics of cationic components during polyphosphate accumulation were investigated in conjunction with transcriptome analysis. Rhizophagus sp. HR1 was grown with Lotus j...

The amount of polyphosphate in the intraradical and extraradical hyphae of Gigaspora margarita was estimated from successive extractions with trichloroacetic acid (TCA), EDTA, and phenol-chloroform (PC). In the intraradical hyphae, most of the polyphosphate was present in TCA- and EDTA-soluble (short-chain and long-chain) fractions, whereas most of the polyphosphate in the extraradical hyphae w...

Abstract New system compromising of chitosan nanoparticles encapsulated pre-synthesized selenium in the presence 5-fluorouracil was successfully prepared and used for cancer antiproliferation. Selenium were synthesized using ascorbic acid as reducing agent under mild condition. Chitosan via ionic gelation technique sodium tri-polyphosphate. Characterization carried out FTIR, TEM, XRD, TGA dynam...