Protein methylase I (S-adenosyl-L-methionine:protein (arginine) N-methyltransferase; EC 2.1.1.23) has been purified from wheat germ approximately 90-fold with a yield of 160%. This unusually high yield of the enzyme activity suggested the presence of an inhibitor. Subsequently, this inhibitor was purified to homogeneity and was found to be identical with adenosine; W absorption spectrum, retent...

The sulfated glycosaminoglycans, such as keratan sulfate and chitin sulfate having 3-hydroxy free N-acetyl-beta-D-glucosaminyl residues as constituents, reacted with wheat germ agglutinin and Solanum tuberosum agglutinin by sugar-specific interaction. The glycosaminoglycans showed different inhibitory activities to the hemagglutination reaction of these lectins and keratan sulfate and its modif...

conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

a newly developed natural rubber deproteinization process produces deproteinized natural rubber (dpnr) wastewater as an intermediate product containing a high concentration of sodium dodecyl sulfate (sds) and rubber. in this study, a novel process to recover the residual rubber and energy as methane from dpnr wastewater was developed. as a pretreatment, sds and residual rubber in dpnr wastewate...

In a previous publication (Fraenkel & Blewett, 1943) it has been shown that a number of insects which normally live on wholemeal flour or other cereal products grow badly or not at all on flour thoroughly extracted with chloroform unless a sterol, e.g. cholesterol, is added to the diet. For several beetle larvae the diet becomes optimal upon addition of a pure sterol, but for the caterpillars o...

The effect of different concentrations of the anionic surfactant sodium dodecyl sulfate (SDS) on biodegradation of diesel oil was assessed during 32 days at 10 degrees C, under simulated environmental conditions, in liquid culture and in an alpine soil. Low SDS concentrations (50-100 mg l-1) significantly enhanced oil biodegradation by a psychrotrophic inoculum in liquid culture, whereas higher...

We have examined the protein components of Sprague-Dawley rat seminal vesicle secretion (SVS) under denaturing conditions by sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Five major components are consistently resolved, which we have referred to as SVS I to SVS V in order of increasing mobility. SVS I to SVS III are relatively insoluble under physiological conditions, but this is no...