A borohydride reduction method was used to identify the phosphorylated amino acid in the phospho-enzyme of the red beet (Beta vulgani L.) plasma membrane ATPase. Plasma membrane fractions were phosphorylated with unlabeled ATP in the presence of MgSO4 at pH 6.5 and then treated with sodium l3Hlborohydride. The borohydride-treated samples were subjected to hydrolysis in 6 normal HCI at 110°C for...

This work is aimed at preparation of small silver nanoparticles (Ag NPs) with various sizes using two-step preparation method. The silver bromide nanoparticles were prepared in the first step and they were reduced to metal silver nanoparticles in the second step. Size of prepared silver nanoparticles was controlled by using of several modifiers. Two surfactants (SDS and Tween 80) and two natura...

The performance of an alkaline fuel cell is investigated using three different fuels, e g., methanol, ethanol and sodium borohydride. Pt/C/Ni was used as anode whereas Mn/C/Ni was used as standard (Electro-Chem-Technic, UK) cathode for all the fuels. Thus, the alkaline fuel cell is used for multi-fuel system. Fresh mixture of electrolyte, potassium hydroxide (5M), and fuel (2M) was fed to and w...

Correction statement In the Methods section of our published article [1], we mentioned that “we developed a novel in situ means of preparing stabilized Au or Ag NPs...”. However, we have recently noticed that the similar process has been reported by Moreno et al. [2]. Hence, we correct the statement in our manuscript by crediting the work by Moreno et al. [2]: [Before Correction] “We developed ...

An enzyme from Vibrio 01 catalyzing cis-tram isomerization of maleylacetone has been identified and pursed. The enzyme has a molecular weight of about 35,000 and appears to be a single unit. Its density is low compared to other proteins. Glutathione is specifically required as a coenzyme. Treatment of the enzyme with sodium borohydride in the presence of substrate and GSH leads to increased act...

A new method for identification and characterization of an acyl phosphate linkage in phosphorylated proteins is presented. The method involves reductive cleavage of the acyl phosphate bond with sodium [3H]borohydride to form a labeled aminohydroxy acid residue. [3H]Borohydride reduction of the phosphorylated (Ca2+, Mg2+)-adenosine triphosphatase of sarcoplasmic reticulum, followed by analysis o...