Sorghum (Sorghum bicolor L. Moench) is a native grass species of the arid and semi-arid regions of Africa (Kimber 2000). Sorghum is a genetically diverse crop and contains some genotypes that have a pigmented testa and therefore contain tannins (Rooney et al 1980; Rooney and Miller 1982). Such lines have the dominant B1_B2_ genes and are the only sorghum types with tannins (Blakeley et al 1979)...

A theoretical analysis of improving the separation power of size exclusion chromatography by decreasing flowrate is conveyed. The variance of the elution curves is larger than expected form estimated dispersity in molecular weight, Mw/Mn. The shape of the elution curves depends strongly on experimental conditions. When the experimental conditions are approaching those to the ideal separation, j...

The compositions of paradoxin and taipoxin (PDx and TPx, respectively) were investigated using size exclusion chromatography (SEC) and nano-electrospray ionization mass spectrometry (nano-ESI-MS). The elution profiles from size exclusion chromatography of the venoms from Oxyuranus microlepidotus and Oxyuranus scutellatus were similar. Fractions corresponding to the trimeric toxins were treated ...

Insulin is a small polypeptide hormone that controls blood glucose homeostasis. Genetic engineering techniques have enabled biopharma companies to develop diverse, long-acting insulin analogs. There is no pharmacopeia method available for the analysis of insulin analogs. An SEC method identifying innovator and biosimilar insulin analog, following a draft EP method, was developed using an Agilen...

Kinetic size exclusion chromatography with mass spectrometry detection (KSEC-MS) is a solution-based label-free approach for studying kinetics of reversible binding of a small molecule to a protein. Extraction of kinetic data from KSEC-MS chromatograms is greatly complicated by the lack of separation between the protein and protein-small molecule complex. As a result, a sophisticated time-consu...

Different degrees of glycosaminoglycan sulfation result in their different charge densities. The charge density differences impact their migration behavior in polyacrylamide gel electrophoresis and size exclusion chromatography, two of the most common methods for determining relative molecular masses of polysaccharides. In this study, we investigated the feasibility of using commercially availa...

The retention behaviour of reference proteins on commercial siliceous size-exclusion supports was studied. Sorption was observed on both surface modified and unmodified supports. When sodium dodecylsulfate was added to the aqueous mobile phase, normal elution patterns were found. With this system, proteins, such as those isolated from different alfalfa genotypes, may be compared rapidly. Compar...

BACKGROUND Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of contaminants such as protein aggregates and incomplete separation of vesicles from lipoproteins, respectively. AIM To develop a single-step protocol...

Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC). Chromatograms are fitted by Exponentially Modified Gaussian functions (EMG) and mapping of band broadening is obtained for different column sets. Interpretation of the skewing of the chromatogra...

Exosomes are a subset of extracellular vesicles (EVs) that have important roles in intercellular communication. They contain and carry bioactive molecules within their membranes which are delivered to target cells. Reproducible isolation and enrichment of these exosomes will aid in evaluation of cellular communication. We present an approach that involved the pre-processing of plasma, combined ...