Ecuador is the leading producer and exporter of fine cocoa, with plantations over 80 years old, preserving distinctive aroma flavor characteristics. The research objective was to screen genetic variability a collection National cocoa from Ecuador's traditional growing areas, denominated as Centennial Cocoa Plants (CCNC). This germplasm 243 accessions analyzed 20 microsatellites (SSR) markers. D...

UNLABELLED PREMISE OF THE STUDY Microsatellite primers were developed for an indigenous fruit tree, Parkia biglobosa, as a tool to study reproductive biology and population structure. Here we use the primers to determine the number of fathers per pod. • METHODS AND RESULTS Microsatellite loci were enriched in a genomic sample and isolated using pyrosequencing. Eleven primer pairs were char...

PREMISE OF THE STUDY Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged ...

Familial hypertrophic cardiomyopathy is a genetically and phenotypically heterogeneous disease caused by mutations in seven sarcomeric protein genes. It is known to be transmitted as an autosomal dominant trait with rare de novo mutations.A French family in which two members are affected by hypertrophic cardiomyopathy was clinically screened with electrocardiography and echocardiography. Geneti...

Multiple DNA microsatellites with frequent loss of heterozygosity (LOH) in melanomas have been demonstrated. The finding that free DNA is enriched in blood of melanoma patients prompted studies to determine whether tumor-specific DNA, such as DNA microsatellites exhibiting LOH, can be detected in blood and have clinical use. In this study, 57 advanced and 19 early clinically staged melanoma pat...

microsatellite markers were used to identify informative markers associated with traits lattice shape of pod, belly shape in pod, pod length, pod width, grain number per pod, grain weight, grain weight/pod weight, grain length, grain width, pod number per plant, grain number per plant, pod weight per plant, grain weight per plant in peanut. thirteen ssr primer pairs amplified 80 alleles among 6...

Abstract. Sukri A, Dewi IN, Primawati SB, Wangiyana IGAS, Muttaqin Z, Winaya A. 2022. Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint. Biodiversitas 23: 4153-4159. (Equus caballus) is a resource that must be preserved. horses have significant economic, social, and cultural role. Information on status needed as step in developing long-term cons...

PURPOSE LOH at the p53 locus has been reported to be associated with esophageal squamous cell carcinogenesis. The aim of this study is to identify potential mechanisms resulting in LOH around the p53 locus in its carcinogenesis. EXPERIMENTAL DESIGN We investigated 10 esophageal cancer cell lines and 91 surgically resected specimens, examining them for LOH at the p53 locus on chromosome 17. We...

the present study describes approaches for the identification of individual beech trees using maternal tissues from their seeds or fruits. four microsatellite markers were used for genetic analysis of seedlots from fagus orientalis lipsky, a highly out-crossing tree species. seeds from 11 single-tree harvests belonging to one population, (7 seeds from each), as well as non-paranchymatic materna...

background: retinoblastoma is the most common intraocular tumor in childhood and mutation in the rb1 gene will trigger the tumorigenesis. so far, a wide range of the mutations along the length of rb1 gene have been reported. however, some could not be detected by common detection methods. in such condition, linkage analysis using microsatellite markers is suggested to trace unknown rb1 mutation...