T-cell activation gene 3 (TCA3) encodes a beta-chemokine that is transcriptionally regulated in mast cells; the gene has a functional NF-kappaB element at positions -194 to -185. The 5'-flanking region of this gene is also known to have a negative regulatory region between -2057 and -1342. To characterize the negative regulatory elements (NREs), this region was sequenced and then digested by Hi...

Ventilation systems installed in buildings usually generate low-frequency noise because the passive silencers commonly used to attenuate the ventilation noise are not effective in the lowfrequency range. A method proven to effectively reduce low-frequency noise in a wide variety of applications is active noise control (ANC). A feedforward ANC system applied to duct noise normally uses a referen...

Transposable elements are driving forces for establishing genetic innovations such as transcriptional regulatory networks in eukaryotic genomes. Here, we describe a silencer situated in the last 300 bp of the Mos1 transposase open reading frame (ORF) which functions in vertebrate and arthropod cells. Functional silencers are also found at similar locations within three other animal mariner elem...

Transcriptional regulation of the plasminogen activator inhibitor type-2 (PAI-2) gene appears to be an important factor in the response of mononuclear phagocytes to inflammation. We have investigated here the molecular basis for PAI-2 synthesis in monocytic cells by reporter gene deletion analysis. A DNA fragment containing 5.1 kb of 5' flanking region through to the start of the second exon wa...

The modification of transcriptional regulation has become increasingly appreciated as a major contributor to morphological evolution. However, the role of negative-acting control elements (e.g. silencers) in generating morphological diversity has been generally overlooked relative to positive-acting "enhancer" elements. The highly variable body coloration patterns among Drosophilid insects repr...

Aromatase has been shown to be expressed at a higher level in human breast cancer tissue than in normal breast tissue, by means of enzyme activity measurement, immunocytochemistry, and RT-PCR analysis. Cell culture including MCF-7 breast cancer cells, animal experiments using aromatase-transfected breast cancer cells, and transgenic mouse studies have demonstrated that estrogen production in si...

The murine homeobox gene Cdx-1 is postulated to play an important role in the process of cellular commitment in the intestinal epithelium based upon its graded, tissue-specific expression pattern in the adult animal. We have isolated and sequenced both cDNA and genomic clones in order to understand how this expression pattern is regulated and to determine the amino acid sequence of the encoded ...

This work presents a mathematical approach based on the point collocation technique to compute the transmission loss of perforated dissipative silencers with transversal temperature gradients and mean flow. Three-dimensional wave propagation is considered in silencer geometries with arbitrary, but axially uniform, cross section. To reduce the computational requirements of a full multidimensiona...